CAJ | 학술논문

目的研究早期瘢痕组织β转化生长因子(transforming growth factor β,TGF-β)的变化,以探索TGF-β在增生性瘢痕形成中的作用,并利用瘢痕水泡液作为研究增生性瘢痕的新途径.方法采用酶联免疫吸附法(ELISA)检测大面积深度烧伤患者愈合后瘢痕增生早期(≤90 d)的水泡液和血液TGF-β1的含量,动态监测瘢痕早期组织TGF-β1的变化,并与正常皮肤组织水泡液和血液对照.结果大面积深度烧伤愈合后瘢痕形成早期患者血液和正常皮肤水泡液TGF-β1的含量与正常人血液比较无升高.均低于本试剂盒最低可检测阈值(15.6 pg/L,P>0.01);增生性瘢痕水泡液TGF-β1含量显著高于其血液和正常皮肤水泡液(P均<0.01),而且瘢痕增生早期(1～3个月)组织TGF-β1逐渐升高(P<0.01).结论瘢痕形成早期组织TGF-β含量增高可能是瘢痕增生的重要因素;瘢痕水泡液可作为研究增生性瘢痕的新途径.
목적 연구조기반흔조직β전화생장인자(transforming growth factor β,TGF-β)적변화,이탐색TGF-β재증생성반흔형성중적작용,병이용반흔수포액작위연구증생성반흔적신도경.방법 채용매련면역흡부법(ELISA)검측대면적심도소상환자유합후반흔증생조기(≤90 d)적수포액화혈액TGF-β1적함량,동태감측반흔조기조직TGF-β1적변화,병여정상피부조직수포액화혈액대조.결과 대면적심도소상유합후반흔형성조기환자혈액화정상피부수포액TGF-β1적함량여정상인혈액비교무승고.균저우본시제합최저가검측역치(15.6 pg/L,P>0.01);증생성반흔수포액TGF-β1함량현저고우기혈액화정상피부수포액(P균<0.01),이차반흔증생조기(1～3개월)조직TGF-β1축점승고(P<0.01).결론 반흔형성조기조직TGF-β함량증고가능시반흔증생적중요인소;반흔수포액가작위연구증생성반흔적신도경.
Objective To investigate of the tissue TGF-β changes at early stage of hypertrophic scar formation and the value of scar blisters in hypertrophic scar.Methods The TGF-β1 content in the blister fluid and the blood were quantified with ELISA,patients(n=15)with hypertrophy scar after depth burn were included,three time point(each n=5)on early　stage(<3 months)of hypertrophy scar formation were monitored.and normal skin blister fluid and the blood(n=5)was used as control.Results The serum TGF-β1 in the both hypertrophic scar patients and normal skin group was not elevated(P>0.01),the TGF-β1 in the blister of normal skin was also not elevated(P>0.01),but TGF-β1 level in the scarblisters hypertrophic scar was elevated significantly[<60 d(158.5±69.8)pg/L,60-90 d,(181.1±40.1)pg/L,>90 d,(534.4±125.9)pg/L,P<0.01] and higher than the normal skin blister and the blood(P<15.6 pg/L.P<0.01),the increased TGF-β1 1evel in the hypertrophic scar blisters were persisted for at least three months.the TGF-β1 level of scar blister on the 3th month of hypertrophic scar formation reached a peak [(534.4±125.9)pg/L,P<0.01].Conclusions The data in this study indicates that TGF-β production at the early stage of hypertrophic scar formation is increased and may play an important role in scar formation;scar blisters is a valuable approach in hypertrophic scar study.