肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
4期
223-226
,共4页
蒋晓东%宋大安%吴瑾%黎世秋
蔣曉東%宋大安%吳瑾%黎世鞦
장효동%송대안%오근%려세추
放射治疗%125I粒子植入%动物,实验
放射治療%125I粒子植入%動物,實驗
방사치료%125I입자식입%동물,실험
Radiotherapy%125I seed brachytherapy%Animals,laboratory
目的 研究125I粒子植入联合放射治疗对A549裸鼠移植瘤生长及乏氧诱导因子-1α(HIF-1α)表达的影响.方法 应用裸鼠建立肺腺癌A549移植瘤模型,待平均瘤体积达到(300±50)mm3时40只裸鼠随机分成4组(每组10只),对照组:不做任何处理;单纯放射治疗组:第1天予单次外照射,6 MV-X线,10 Gy;125I粒子植入组:肿瘤中心植入1枚活度27.75 MBq的125I粒子;放疗加125I粒子植入组(联合治疗组):第1天在肿瘤中心植入1枚活度27.75 MBq的125I粒子的同时予单次外照射,6MV-X线,10Gy.治疗后共观察15 d,绘制肿瘤生长曲线,测量各组肿瘤体积并计算抑瘤率,分别用免疫组织化学及蛋白免疫印迹方法测定HIF-1α的表达.结果 治疗第8天起,瘤体积联合治疗组(209±21)mm3与对照组(322±30)mm3相比差异有统计学意义(t=46.4,P=0.000).第15天后,单纯放射治疗组、125I粒子植入组、联合治疗组抑瘤率依次为45.9%、44.4%、69.4%,联合治疗组与单纯放射治疗组比较差异有统计学意义(t=52.03,P=0.000),联合治疗组与125I粒子植入组比较差异有统计学意义(t=53.98,P=0.000),单纯放射治疗组和125I粒子植入组相比,差异无统计学意义(t=0.591,P=0.596).联合治疗组与其他各组相比HIF-1α改变差异均无统计学意义(P=0.342).结论 放射治疗联合125I粒子植入能显著抑制转染人类肺腺癌A549裸鼠移植肿瘤的生长,但未抑制肿瘤细胞HIF-1α的表达.
目的 研究125I粒子植入聯閤放射治療對A549裸鼠移植瘤生長及乏氧誘導因子-1α(HIF-1α)錶達的影響.方法 應用裸鼠建立肺腺癌A549移植瘤模型,待平均瘤體積達到(300±50)mm3時40隻裸鼠隨機分成4組(每組10隻),對照組:不做任何處理;單純放射治療組:第1天予單次外照射,6 MV-X線,10 Gy;125I粒子植入組:腫瘤中心植入1枚活度27.75 MBq的125I粒子;放療加125I粒子植入組(聯閤治療組):第1天在腫瘤中心植入1枚活度27.75 MBq的125I粒子的同時予單次外照射,6MV-X線,10Gy.治療後共觀察15 d,繪製腫瘤生長麯線,測量各組腫瘤體積併計算抑瘤率,分彆用免疫組織化學及蛋白免疫印跡方法測定HIF-1α的錶達.結果 治療第8天起,瘤體積聯閤治療組(209±21)mm3與對照組(322±30)mm3相比差異有統計學意義(t=46.4,P=0.000).第15天後,單純放射治療組、125I粒子植入組、聯閤治療組抑瘤率依次為45.9%、44.4%、69.4%,聯閤治療組與單純放射治療組比較差異有統計學意義(t=52.03,P=0.000),聯閤治療組與125I粒子植入組比較差異有統計學意義(t=53.98,P=0.000),單純放射治療組和125I粒子植入組相比,差異無統計學意義(t=0.591,P=0.596).聯閤治療組與其他各組相比HIF-1α改變差異均無統計學意義(P=0.342).結論 放射治療聯閤125I粒子植入能顯著抑製轉染人類肺腺癌A549裸鼠移植腫瘤的生長,但未抑製腫瘤細胞HIF-1α的錶達.
목적 연구125I입자식입연합방사치료대A549라서이식류생장급핍양유도인자-1α(HIF-1α)표체적영향.방법 응용라서건립폐선암A549이식류모형,대평균류체적체도(300±50)mm3시40지라서수궤분성4조(매조10지),대조조:불주임하처리;단순방사치료조:제1천여단차외조사,6 MV-X선,10 Gy;125I입자식입조:종류중심식입1매활도27.75 MBq적125I입자;방료가125I입자식입조(연합치료조):제1천재종류중심식입1매활도27.75 MBq적125I입자적동시여단차외조사,6MV-X선,10Gy.치료후공관찰15 d,회제종류생장곡선,측량각조종류체적병계산억류솔,분별용면역조직화학급단백면역인적방법측정HIF-1α적표체.결과 치료제8천기,류체적연합치료조(209±21)mm3여대조조(322±30)mm3상비차이유통계학의의(t=46.4,P=0.000).제15천후,단순방사치료조、125I입자식입조、연합치료조억류솔의차위45.9%、44.4%、69.4%,연합치료조여단순방사치료조비교차이유통계학의의(t=52.03,P=0.000),연합치료조여125I입자식입조비교차이유통계학의의(t=53.98,P=0.000),단순방사치료조화125I입자식입조상비,차이무통계학의의(t=0.591,P=0.596).연합치료조여기타각조상비HIF-1α개변차이균무통계학의의(P=0.342).결론 방사치료연합125I입자식입능현저억제전염인류폐선암A549라서이식종류적생장,단미억제종류세포HIF-1α적표체.
Objective To study the inhibitory effects of radiotherapy and 125I seed brachytherapy on the growth of transplanted human lung cancer cell line A549 in nude mice and the impact of HIF-1αexpression after therapy.Methods Forty nude mice bearing human lung cancer cell line A549 were randomly divided into control group,radiotherapy group,125I seed brachytherapy group and radiotherapy + 125I seed group when tumor volume achieved (300±50) mm3.The tumor growth was observed and the alteration of tumor size was calculated at different time.On 15th day,the expression of HIF-1α was detected by immunohistochemistry and western blot.Results When eighth day after treatment,compared with the control group,the tumor volume of the combined treatment group was significantly smaller (t = 46.4,P ＜0.05).After fifteenth day after treatment,compared with control group,the group of radiotherapy,125I seed brachytherapy and radiotherapy + 125I seed gained the tumor control rate of 45.9 %,44.4 %,69.4 % respectively.Compared with other groups,the change of expression of HIF-1α in the combined treatment group was not significant (P ＞0.05).Conclusion Radiotherapy combined with 125I seed brachytherapy can inhibit the growth of transplanted human lung cancer cell line A549 in nude mice,and the tumor regression can be observed in early stage.But in our study,the expression of HIF-1α in tumors cannot be inhibited by 125I seed.
