CAJ | 학술논문

[目的]对苦瓜叶进行了提取分离,找出苦瓜叶中的抗黄足黑守瓜取食成分,阐明苦瓜叶抗虫机理.[方法]用不同极性有机溶剂分别对苦瓜叶进行提取、浓缩,得到3种有机溶剂提取物,对黄足黑守瓜进行了选择性与非选择性生物测定. [结果] 3种提取物都随浓度的增加,选择性拒食作用与非选择性拒食作用增强,氯仿提取物对黄足黑守瓜的拒食作用最强,在处理浓度为8 mg/ml,48 h后其选择性拒食率与非选择性拒食率分别为100%和96.47%.进一步用100～200目硅胶柱层析法对氯仿提取物进行组分分离,并进行生物测定,发现各组分对黄足黑守瓜的拒食作用差别很大, 4组分、9组分显示出非常好的拒食作用,其拒食率在处理24和48 h后均达100%.[结论] 苦瓜叶中含有对黄足黑守瓜具有忌避作用的次生代谢物质,从而起到忌避其成虫前来取食的作用.
[목적]대고과협진행료제취분리,조출고과협중적항황족흑수과취식성분,천명고과협항충궤리.[방법]용불동겁성유궤용제분별대고과협진행제취、농축,득도3충유궤용제제취물,대황족흑수과진행료선택성여비선택성생물측정. [결과] 3충제취물도수농도적증가,선택성거식작용여비선택성거식작용증강,록방제취물대황족흑수과적거식작용최강,재처리농도위8 mg/ml,48 h후기선택성거식솔여비선택성거식솔분별위100%화96.47%.진일보용100～200목규효주층석법대록방제취물진행조분분리,병진행생물측정,발현각조분대황족흑수과적거식작용차별흔대, 4조분、9조분현시출비상호적거식작용,기거식솔재처리24화48 h후균체100%.[결론] 고과협중함유대황족흑수과구유기피작용적차생대사물질,종이기도기피기성충전래취식적작용.
[Objective] The mechanism of Momordica charantia L. leaf resisting pest was explored through the analysis of the component in its leaf that was eaten by Aulacophora lewisii Baly adult through the research on the extracts from the leaf. [Method] Three extracts from Momordica charantia L. leaf were produced with the organic solvents with different polarities. The test in the biological choice and non-choice of the pest to them was studied. [Results] The results showed that the anti-feeding behavior of the pest in both choice and non-choice was gradually strong with the increment of the extracts concentration; and the anti-feeding behavior of the pest appeared in the extract from chloroform solvent and the most intensity of anti-feeding behavior of the pest in both choice and non-choice was in the treatment with the concentration of 8 mg/ml for 48 hours, which was 100% and 96.47% respectively. The anti-feeding behavior of the pest in both choice and non-choice was different in the biological test with the treatment that the extract from chloroform solvent was further isolated by 100-200 mesh silica gel column chromatography. The better anti-feeding behavior of the pest appeared in the treatment of the fraction 4 and fraction 9, which was 100% in the 24 and 48 hours' treatment, respectively. [Conclusion] The secondary metabolites in Momordica charantia L. leaf had the repellent effect on Aulacophora lewisii Baly.