CAJ | 학술논문

目的:研究荷瘤小鼠来源的髓样抑制细胞(Myeloid derived suppressor cell,MDSC)在肿瘤免疫抑制中的作用机理.方法:用Percoll分离法从荷瘤小鼠的脾脏和骨髓中分离Gr-1~+CDllb~+MDSC;用流式细胞术检测MDSC对T细胞增殖的抑制作用;分别用生化法和ELISA技术检测MDSC体外培养上清中抑制性因子NO、ROS和IL-10、TGF-β的含量.结果:MDSC在荷瘤小鼠的脾脏和骨髓中聚集增多,且其在骨髓中所占的比例显著高于脾脏;MDSC可以明显抑制脾脏细胞的增殖,体外培养6小时的MDSC可以分泌大量NO、ROS和IL-10、TGF-β.结论:本实验进一步证实MDSC可以通过分泌大量NO、ROS和IL-10、TGV-β抑制T细胞增殖.
목적:연구하류소서래원적수양억제세포(Myeloid derived suppressor cell,MDSC)재종류면역억제중적작용궤리.방법:용Percoll분리법종하류소서적비장화골수중분리Gr-1~+CDllb~+MDSC;용류식세포술검측MDSC대T세포증식적억제작용;분별용생화법화ELISA기술검측MDSC체외배양상청중억제성인자NO、ROS화IL-10、TGF-β적함량.결과:MDSC재하류소서적비장화골수중취집증다,차기재골수중소점적비례현저고우비장;MDSC가이명현억제비장세포적증식,체외배양6소시적MDSC가이분비대량NO、ROS화IL-10、TGF-β.결론:본실험진일보증실MDSC가이통과분비대량NO、ROS화IL-10、TGV-β억제T세포증식.
Objective:To study the mechanisms of immune suppression mediated by Gr-1+CDllb+ myeloid derived suppressor cells (Gr-1~+CDllb~+MDSC)from tumor-bearing mice.Methods:Gr-1~+CDllb~+MDSC recruited into spleen and bone marrow of tumor-bearing mice were purified by Percoll,and suppression mediated by MDSC on T cell proliferation from spleen of naive mice was detected by flow cytometry with CSFE and FTTC-anti CD3 staining,and NO,ROS,IL-10 and TGF-β in the supematant of MDSC were detected by Griess and ELISA.Results:There were much more Gr-1~+CDllb~+MDSCs in spleen and bone marrow from tumor-bearing mouse than those of naive mouse,and suppression on T cell proliferation mediated by MDSC from tumor beating mouse was significantly increased,and there were much more NO,ROS,IL-10 and TGF-βin the supematant of these MDSC than that from naive mouse.Conclusion:MDSC from tumor-bearing mice secreted hish level of NO,ROS,IL-10 and TGF-βto induce immune suppression,and inhibite the proliferation of T cells.