CAJ | 학술논문

背景:人脂肪间充质干细胞具有增殖和多向分化的能力,作为一种新型的干细胞已被广泛应用于组织工程研究,并在其已有的和潜在的生物治疗应用中发挥着重要作用.目的:通过检索相关文献,综述人脂肪间充质干细胞在组织工程和细胞治疗中的应用及存在的问题.方法:应用计算机检索CNKI和PubMed数据库中1960-01/2010-01关于人脂肪间充质干细胞应用的相关文章,检索词分别为"人脂肪间充质干细胞;分离;分化;免疫表型;应用"和"human adipose tissue-derived stromal cells;isolation;differentiation;immune phenotype;application",语言分别设定为中文和英文.选择内容与人脂肪间充质干细胞生物特性和应用相关的文章,同一领域文献则选择近期发表或发表在权威杂志的文章,排除重复类文章. 结果与结论:收集到81篇相关文献,排除不符合标准的文献,共纳入57篇符合标准的文献.结果表明,人脂肪间充质干细胞具有许多特性,包括间充质干细胞潜在的增殖能力和适当条件下的多向分化能力.人脂肪间充质干细胞,不仅可以应用于组织的修复,而且在细胞免疫调节和基因治疗中均发挥重要作用.但人脂肪间充质干细胞的应用过程中仍存在许多问题.随着人脂肪间充质干细胞研究的深入,更多的生物学特性将会发现,人脂肪间充质干细胞在组织修复,细胞治疗,移植以及基因治疗中的应用前景会更加广阔.
배경:인지방간충질간세포구유증식화다향분화적능력,작위일충신형적간세포이피엄범응용우조직공정연구,병재기이유적화잠재적생물치료응용중발휘착중요작용.목적:통과검색상관문헌,종술인지방간충질간세포재조직공정화세포치료중적응용급존재적문제.방법:응용계산궤검색CNKI화PubMed수거고중1960-01/2010-01관우인지방간충질간세포응용적상관문장,검색사분별위"인지방간충질간세포;분리;분화;면역표형;응용"화"human adipose tissue-derived stromal cells;isolation;differentiation;immune phenotype;application",어언분별설정위중문화영문.선택내용여인지방간충질간세포생물특성화응용상관적문장,동일영역문헌칙선택근기발표혹발표재권위잡지적문장,배제중복류문장. 결과여결론:수집도81편상관문헌,배제불부합표준적문헌,공납입57편부합표준적문헌.결과표명,인지방간충질간세포구유허다특성,포괄간충질간세포잠재적증식능력화괄당조건하적다향분화능력.인지방간충질간세포,불부가이응용우조직적수복,이차재세포면역조절화기인치료중균발휘중요작용.단인지방간충질간세포적응용과정중잉존재허다문제.수착인지방간충질간세포연구적심입,경다적생물학특성장회발현,인지방간충질간세포재조직수복,세포치료,이식이급기인치료중적응용전경회경가엄활.
BACKGROUND: Human adipose tissue-derived stromal cells (ADSCs) possess exert proliferation and multi-directional ability. As a novel stem cell, it has been widely utilized in tissue engineering and plays an important role in biological and potential therapeutic applications.OBJECTIVE: To review the research progress, applications and existing problems of human ADSCs in tissue engineering and cell therapy by retrieving relevant publications. METHODS: PubMed and CNKI databases were undertaken to identify the relevant articles published from January 1960 to January 2009 with the key words of "human adipose tissue-derived mesenchymal cells, isolation, differentiation, immune phenotype, application" both in English and Chinese. The articles relate to biological characteristics and applications of ADSCs were selected. In the same field, the documents published earlier or in the authoritative journals were preferred, and the repetitive studies were excluded. RESULTS AND CONCLUSION: A total of 81 documents were searched by computer, and 57 references were included in the final analysis. The results demonstrated that, human ADSCs share many characteristics, including the high proliferative potential and exhibiting the exert ability to undergo multilineage differentiation under appropriate conditions. Human ADSCs can not only utilize for tissue repairing, but also for cell immune modulation and gene therapy. However, there are still problems in its application. With the development of research on human ADSCs, their biological characteristics will be revealed, and their application in tissue repairing, cell therapy, transplantation, as well as gene therapy must be hold a great promise.