pEGFP/A2M(FP6)重组质粒转染小鼠胚胎神经干细胞的实验研究
pEGFP/A2M(FP6)중조질립전염소서배태신경간세포적실험연구
Transfection of pEGFP/A2M(FP6) Plasmid Vectors into Mouse Embryonic Neural Stem Cells
  • 万方数据
    神经损伤与功能重建 신경손상여공능중건
    NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
    2011年 1期 19-23 ,共5页

    武强%李露斯%范文辉%黎红华%崔敏%吴非%徐志鹏%濮捷%杨梅

    무강%리로사%범문휘%려홍화%최민%오비%서지붕%복첩%양매

    pEGFP/A2M(FP6)%Nucleofector转染%神经干细胞 pEGFP/A2M(FP6)%Nucleofector轉染%神經榦細胞
    pEGFP/A2M(FP6)%Nucleofector전염%신경간세포
    目的:观察携带pEGFP/A2M(FP6)重组质粒的神经干细胞(NSCs)的生物学特性.方法:培养NSCs,采用Nucleofector转染方法将pEGFP/A2M(FP6)重组质粒转染至NSCs,利用其携带的绿色荧光和RT-PCR观察其转染情况,免疫组织化学法检测A2M(FP6)蛋白表达.结果:Nucleofector转染方法可成功高效地将pEGFP/A2M(FP6)重组质粒转染至NSCs,且对NSCs的增殖、分化无明显影响,转染细胞出现A2M(FP6)的表达.结论:携带pEGFP/A2M(FP6)质粒的NSCs仍具有增殖、分化特性,并具有合成A2M(FP6)的能力.
    목적:관찰휴대pEGFP/A2M(FP6)중조질립적신경간세포(NSCs)적생물학특성.방법:배양NSCs,채용Nucleofector전염방법장pEGFP/A2M(FP6)중조질립전염지NSCs,이용기휴대적록색형광화RT-PCR관찰기전염정황,면역조직화학법검측A2M(FP6)단백표체.결과:Nucleofector전염방법가성공고효지장pEGFP/A2M(FP6)중조질립전염지NSCs,차대NSCs적증식、분화무명현영향,전염세포출현A2M(FP6)적표체.결론:휴대pEGFP/A2M(FP6)질립적NSCs잉구유증식、분화특성,병구유합성A2M(FP6)적능력.
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