CAJ | 학술논문

介绍了一种微电极阵列(MEA),该MEA包含60个独立的电极,其中59个为工作电极,一个为参考电极.工作电极成对排列,每一对工作电极分别包含一个激励电极和一个探测电极.结合外围电路,每一对工作电极的组合都可以实现对电极表面培养的生物组织或切片进行4种状态(激励、探测、同时激励和探测、隔离)的控制.MEA采用PCB工艺制作完成,总面积为49 mm×49 mm.采用LCR测试仪,在20 Hz～10 kHz,50 mV交流信号下对工作电极进行了阻抗测试,测试在0.9％的NaCl溶液中进行.测试结果表明,频率在500～1 000 Hz之间时,工作电极的阻抗在200 ～3 kΩ之间,电极的阻抗幅值与频率成反比.采用MEA进行了蟾蜍离体坐骨神经干电信号激励与探测实验,及坐骨神经干之间的神经信号再生实验.实验结果表明,MEA可实现对体外培养的生物组织或切片进行激励、探测、同时激励和探测、隔离4种状态的控制.
개소료일충미전겁진렬(MEA),해MEA포함60개독립적전겁,기중59개위공작전겁,일개위삼고전겁.공작전겁성대배렬,매일대공작전겁분별포함일개격려전겁화일개탐측전겁.결합외위전로,매일대공작전겁적조합도가이실현대전겁표면배양적생물조직혹절편진행4충상태(격려、탐측、동시격려화탐측、격리)적공제.MEA채용PCB공예제작완성,총면적위49 mm×49 mm.채용LCR측시의,재20 Hz～10 kHz,50 mV교류신호하대공작전겁진행료조항측시,측시재0.9％적NaCl용액중진행.측시결과표명,빈솔재500～1 000 Hz지간시,공작전겁적조항재200 ～3 kΩ지간,전겁적조항폭치여빈솔성반비.채용MEA진행료섬서리체좌골신경간전신호격려여탐측실험,급좌골신경간지간적신경신호재생실험.실험결과표명,MEA가실현대체외배양적생물조직혹절편진행격려、탐측、동시격려화탐측、격리4충상태적공제.
A microelectrode array(MEA) is presented,which is composed of 60 independent electrodes with 59 working ones and one reference one,and they are divided into 30 pairs.Except for the reference electrode,each pair consists of one stimulating electrode and one recording electrode.Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation,recording, stimulation and recording simultaneously, and isolation.The state of each pair of working electrodes can be arbitrarily controlled according to actual needs.The MEAs are fabricated in printed circuit board (PCB) technology.The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9％ sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter,and the test signal for the impedance measurement is sinusoidal (AC voltage 50 mV,sweeping frequency 20 Hz to 10 kHz).The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz.The electrode impedance magnitude is inversely proportional to the frequency.Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA.The results show that the MEA can be used for bioelectrical signal stimulation,recording,stimulation and recording simultaneously,and isolation of biological tissues or slices in-vitro.