中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2012年
1期
30-32
,共3页
李星%马刘江%玄永哲%安京华%李香善%石俊
李星%馬劉江%玄永哲%安京華%李香善%石俊
리성%마류강%현영철%안경화%리향선%석준
廿烷五烯酸%A-549细胞系%凋亡
廿烷五烯痠%A-549細胞繫%凋亡
입완오희산%A-549세포계%조망
Eicosapentaenoic acid%A-549 cell lines%apoptosis
目的 观察廿烷五烯酸(EPA)单剂以及与卡铂联合应用对人肺腺癌A-549细胞系增殖和凋亡的影响.方法 分别用100 μg/ml卡铂、80 μg/ml EPA以及100 μg/ml卡铂联合80 μg/ml EPA孵育A-549细胞48 h后,采用MTT法检测药物对A-549细胞增殖的抑制率,HE染色观察细胞形态学,流式细胞术定量分析细胞凋亡率.结果 100 μg/ml卡铂联合80 μg/ml EPA对A-549细胞系的增殖抑制率为85.20％±5.00％,显著高于80 μg/ml EPA( 32.85％±3.00％,P=0.0001)或100 μg/ml卡铂(53.25％±3.00％,P=0.0013)单独的作用.HE染色显示:药物干预后部分A-549细胞出现凋亡形态学改变.卡铂联合EPA组A-549细胞凋亡率为17.05％±4.00％,显著高于单用卡铂组(9.49％±1.00％,P=0.0252).结论 EPA可能具有增强卡铂抑制人肺腺癌A-549细胞系增殖、促进A-549细胞系凋亡的作用.
目的 觀察廿烷五烯痠(EPA)單劑以及與卡鉑聯閤應用對人肺腺癌A-549細胞繫增殖和凋亡的影響.方法 分彆用100 μg/ml卡鉑、80 μg/ml EPA以及100 μg/ml卡鉑聯閤80 μg/ml EPA孵育A-549細胞48 h後,採用MTT法檢測藥物對A-549細胞增殖的抑製率,HE染色觀察細胞形態學,流式細胞術定量分析細胞凋亡率.結果 100 μg/ml卡鉑聯閤80 μg/ml EPA對A-549細胞繫的增殖抑製率為85.20％±5.00％,顯著高于80 μg/ml EPA( 32.85％±3.00％,P=0.0001)或100 μg/ml卡鉑(53.25％±3.00％,P=0.0013)單獨的作用.HE染色顯示:藥物榦預後部分A-549細胞齣現凋亡形態學改變.卡鉑聯閤EPA組A-549細胞凋亡率為17.05％±4.00％,顯著高于單用卡鉑組(9.49％±1.00％,P=0.0252).結論 EPA可能具有增彊卡鉑抑製人肺腺癌A-549細胞繫增殖、促進A-549細胞繫凋亡的作用.
목적 관찰입완오희산(EPA)단제이급여잡박연합응용대인폐선암A-549세포계증식화조망적영향.방법 분별용100 μg/ml잡박、80 μg/ml EPA이급100 μg/ml잡박연합80 μg/ml EPA부육A-549세포48 h후,채용MTT법검측약물대A-549세포증식적억제솔,HE염색관찰세포형태학,류식세포술정량분석세포조망솔.결과 100 μg/ml잡박연합80 μg/ml EPA대A-549세포계적증식억제솔위85.20％±5.00％,현저고우80 μg/ml EPA( 32.85％±3.00％,P=0.0001)혹100 μg/ml잡박(53.25％±3.00％,P=0.0013)단독적작용.HE염색현시:약물간예후부분A-549세포출현조망형태학개변.잡박연합EPA조A-549세포조망솔위17.05％±4.00％,현저고우단용잡박조(9.49％±1.00％,P=0.0252).결론 EPA가능구유증강잡박억제인폐선암A-549세포계증식、촉진A-549세포계조망적작용.
Objective To investigate the effects of eicosapentaenoic acid (EPA) or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549.Methods A-549 cells were cultured by 100 μg/ml carboplatin,80 μg/ml EPA,and their combination for 48 hours.MTT assay was used to determine the effect of EPA,carboplatin,or their combination on the proliferation of human lung cancer cell line A-549.The morphological changes of human lung cancer cell line A-549 were observed using HE staining,and apoptosis of A-549 cells was analyzed by flow cytometry.Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20％ ± 5.00％,which was significantly higher than that of cells cultured with 80 μg/ml EPA (32.85％ ± 3.00％,P =0.0001 ) or 100 μg/ml carboplatin (53.25％ ±3.00％,P =0.0013 ).HE staining showed apoptosis in all three groups,whereas the apoptosis rate reached 17.05％ ± 4.00％ in the combination group,which was significantly higher than that in carboplatin group (9.49％ ± 1.00％,P =0.0252).Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.
