中华航空航天医学杂志
中華航空航天醫學雜誌
중화항공항천의학잡지
CHINESE JOURNAL OF AEROSPACE MEDICINE
2011年
3期
189-193
,共5页
黄立桂%王恩彤%陈伟%龚维熙
黃立桂%王恩彤%陳偉%龔維熙
황립계%왕은동%진위%공유희
运动病%组胺%受体,组胺H1%异丙嗪%大鼠
運動病%組胺%受體,組胺H1%異丙嗪%大鼠
운동병%조알%수체,조알H1%이병진%대서
Motion sickness%Histamine%Receptors,histamine H1%Promethazine%Rats
目的 研究H1受体在大鼠脑于前庭核的表达及其在运动病发生和信号转导过程中的作用.方法 24只健康SD大鼠(200 g~250 g,雌雄各半)依随机数字表法随机分为4组,每组6只.A:对照组,不接受运动刺激和抗运动病药物;B:仅暴露运动刺激组;C:仅接受抗运动病药物组(H1受体阻断剂盐酸异丙嗪,0.25 mg/只,腹腔注射);D:接受运动刺激和抗运动病药物组.通过旋转摆动复合运动刺激诱使大鼠发生运动病,以出现对糖精水的厌饮症状作为运动病发病指标.测定运动刺激后及暴露其他相应实验因素后45 min内各组小鼠0.15%糖精水的饮用量.通过免疫荧光染色观察大鼠脑干前庭核H1受体的分布;蛋白印迹法分析大鼠前庭核层面脑干组织H1受体蛋白的表达,并观察运动刺激和异丙嗪对H1受体表达的影响.结果 各组大鼠平均糖精水饮用量有明显差异(F=346.82,P<0.01).运动刺激组平均糖精水饮用量明显少于对照组,差异有统计学意义(P<0.01).异丙嗪组平均糖精水饮用量与对照组相近(P>0.05).应用异丙嗪后接受运动刺激组的平均糖精水饮用量多于运动刺激组(P<0.01),但仍明显少于对照组及异丙嗪组(P<0.01).免疫荧光染色显示,大鼠脑干前庭核表达有H1受体,运动刺激可使其表达增加,但未受到异丙嗪的明显抑制.蛋白印迹法分析显示,大鼠前庭核层面脑干组织有H1受体蛋白的表达,运动刺激亦使其表达增加,异丙嗪对其表达增加不具有明显的抑制作用.结论 旋转摆动复合运动刺激可有效地诱发大鼠发生运动病.大鼠脑干前庭核存在有H1受体,运动刺激可使其表达增加.异丙嗪具有一定的抗运动病作用,但对运动刺激所诱发的大鼠脑干前庭核H1受体表达增加没有明显的抑制作用.其作用机制可能是与组胺竞争性地结合H1受体而非通过影响H1受体的表达.
目的 研究H1受體在大鼠腦于前庭覈的錶達及其在運動病髮生和信號轉導過程中的作用.方法 24隻健康SD大鼠(200 g~250 g,雌雄各半)依隨機數字錶法隨機分為4組,每組6隻.A:對照組,不接受運動刺激和抗運動病藥物;B:僅暴露運動刺激組;C:僅接受抗運動病藥物組(H1受體阻斷劑鹽痠異丙嗪,0.25 mg/隻,腹腔註射);D:接受運動刺激和抗運動病藥物組.通過鏇轉襬動複閤運動刺激誘使大鼠髮生運動病,以齣現對糖精水的厭飲癥狀作為運動病髮病指標.測定運動刺激後及暴露其他相應實驗因素後45 min內各組小鼠0.15%糖精水的飲用量.通過免疫熒光染色觀察大鼠腦榦前庭覈H1受體的分佈;蛋白印跡法分析大鼠前庭覈層麵腦榦組織H1受體蛋白的錶達,併觀察運動刺激和異丙嗪對H1受體錶達的影響.結果 各組大鼠平均糖精水飲用量有明顯差異(F=346.82,P<0.01).運動刺激組平均糖精水飲用量明顯少于對照組,差異有統計學意義(P<0.01).異丙嗪組平均糖精水飲用量與對照組相近(P>0.05).應用異丙嗪後接受運動刺激組的平均糖精水飲用量多于運動刺激組(P<0.01),但仍明顯少于對照組及異丙嗪組(P<0.01).免疫熒光染色顯示,大鼠腦榦前庭覈錶達有H1受體,運動刺激可使其錶達增加,但未受到異丙嗪的明顯抑製.蛋白印跡法分析顯示,大鼠前庭覈層麵腦榦組織有H1受體蛋白的錶達,運動刺激亦使其錶達增加,異丙嗪對其錶達增加不具有明顯的抑製作用.結論 鏇轉襬動複閤運動刺激可有效地誘髮大鼠髮生運動病.大鼠腦榦前庭覈存在有H1受體,運動刺激可使其錶達增加.異丙嗪具有一定的抗運動病作用,但對運動刺激所誘髮的大鼠腦榦前庭覈H1受體錶達增加沒有明顯的抑製作用.其作用機製可能是與組胺競爭性地結閤H1受體而非通過影響H1受體的錶達.
목적 연구H1수체재대서뇌우전정핵적표체급기재운동병발생화신호전도과정중적작용.방법 24지건강SD대서(200 g~250 g,자웅각반)의수궤수자표법수궤분위4조,매조6지.A:대조조,불접수운동자격화항운동병약물;B:부폭로운동자격조;C:부접수항운동병약물조(H1수체조단제염산이병진,0.25 mg/지,복강주사);D:접수운동자격화항운동병약물조.통과선전파동복합운동자격유사대서발생운동병,이출현대당정수적염음증상작위운동병발병지표.측정운동자격후급폭로기타상응실험인소후45 min내각조소서0.15%당정수적음용량.통과면역형광염색관찰대서뇌간전정핵H1수체적분포;단백인적법분석대서전정핵층면뇌간조직H1수체단백적표체,병관찰운동자격화이병진대H1수체표체적영향.결과 각조대서평균당정수음용량유명현차이(F=346.82,P<0.01).운동자격조평균당정수음용량명현소우대조조,차이유통계학의의(P<0.01).이병진조평균당정수음용량여대조조상근(P>0.05).응용이병진후접수운동자격조적평균당정수음용량다우운동자격조(P<0.01),단잉명현소우대조조급이병진조(P<0.01).면역형광염색현시,대서뇌간전정핵표체유H1수체,운동자격가사기표체증가,단미수도이병진적명현억제.단백인적법분석현시,대서전정핵층면뇌간조직유H1수체단백적표체,운동자격역사기표체증가,이병진대기표체증가불구유명현적억제작용.결론 선전파동복합운동자격가유효지유발대서발생운동병.대서뇌간전정핵존재유H1수체,운동자격가사기표체증가.이병진구유일정적항운동병작용,단대운동자격소유발적대서뇌간전정핵H1수체표체증가몰유명현적억제작용.기작용궤제가능시여조알경쟁성지결합H1수체이비통과영향H1수체적표체.
Objective To investigate the expression of histamine H1 receptors (H1 R) in vestibular nucleus of rat' s brainstem and the underlying role of H1R in motion sickness (MS).Methods Total of 24 healthy Sprague-Dawley rats were randomly divided into four groups,6 rats in each group.A:MS(-)/Drug(-) control group,in which the rats were not exposed to motion stimulus and no anti-motion sickness drug promethazine taking; B:MS(+)/Drug(-) group,only exposed to motion stimulus; C:MS(-)/Drug(+) group,that applied with promethazine but not exposed to motion stimulus; D:MS(+)/Drug (+) group,applied with promethazine (0.25 mg for each rat) 30 minutes before exposing to motion stimulus.MS was induced by a complex motion stimulus and the conditioned taste aversion was used as the behavioral indicator of MS in rats,measuring the intaked volume of 0.15% sodium saccharin solution (SSS) for each rat 45 minutes after motion stimulus.H1 R in the vestibular nucleus of brainstem was examined by immunofluorescence staining.The expression of H1 R proteins in hrainstem tissue at vestibular nucleus level was detected by Western blot.The effects of motion stimulus and anti-MS drug promethazine on the expressions of H1R were evaluated.Results The mean intake of SSS in B group was significantly less than that in A group (F=346.82,P<0.01),which demondtrated that MS was induced by this motion stimulus in the rats.The mean intaked SSS volume (14.8 ml) in C group was similar to that in A group (P>0.05),indicating no significant effect of promethazine on intaking SSS.The mean intaked SSS volume in D group was more than that in B group (P<0.01),but less than that in A group or C group (P<0.01),which indicated that the inhibited intaking of SSS in motion-stimulated rats was improved partially by promethazine.Immunofluorescence staining showed the positive expression of H1R in the vestibular nucleus of brainstem in rats and the expression was enhanced by motion stimulus,but was not significantly affected by promethazine.Western blot analysis showed that H1 R proteins expressed in the brainstem tissues at vestibular nucleus level,which were also increased significantly after motion stimulus,but the increase was not affected by promethazine.Conclusions H1 R exists in vestibular nucleus of brainstem in rats.The expression of H1R is up-regulated by motion stimulus,but is not affected by anti-MS drug promethazine.It indicates that histaminergic system is involved in the development of MS.Promethazine,as a blocker H1R,plays the role of anti-MS by binding H1R rather than by increasing the expression of H1R.