CAJ | 학술논문

目的建立小鼠放射性肠炎模型.方法雌性BALB/c小鼠84只,随机分为对照组(24只)、6 Gy组(28只)及10 Gy组(32只)60Co γ射线对小鼠腹部一次性照射,在照射后3、7、14和21 d检测血象及肝、肾功能,并取肠道组织做病理及一氧化氮(NO)检测.结果照射后3、7 d,6 Gy及10 Gy组WBC、PIJT均较对照组降低;10 Gy组肝功能异常;10 Gy组照射后3 d肠道组织NO水平降低;照射后3、7 d,2个照射组小肠绒毛高度降低,隐窝深度变浅、数目减少,隐窝腔变大,均出现放射性肠炎改变,10 Gy组死亡较多,6 Gy组更能模拟临床放射性肠炎,为研究提供模型.结论 60Co射线一次性腹部照射建立小鼠放射性肠炎模型,6 Gy照射剂量更为合适.
목적 건립소서방사성장염모형.방법 자성BALB/c소서84지,수궤분위대조조(24지)、6 Gy조(28지)급10 Gy조(32지)60Co γ사선대소서복부일차성조사,재조사후3、7、14화21 d검측혈상급간、신공능,병취장도조직주병리급일양화담(NO)검측.결과 조사후3、7 d,6 Gy급10 Gy조WBC、PIJT균교대조조강저;10 Gy조간공능이상;10 Gy조조사후3 d장도조직NO수평강저;조사후3、7 d,2개조사조소장융모고도강저,은와심도변천、수목감소,은와강변대,균출현방사성장염개변,10 Gy조사망교다,6 Gy조경능모의림상방사성장염,위연구제공모형.결론 60Co사선일차성복부조사건립소서방사성장염모형,6 Gy조사제량경위합괄.
Objective To explore the optimum dose for the mouse radiation enteritis model by abdominal irradiation(AR).Methods 84 BALB/c female mice were randomly divided into three groups as control group,6 Gy group and 10 Gy group.For each group,mice were sacrificed at 3,7,14 and 21 days after single abdominal irradiation of Cobalt-60,respectively.The blood was sampled to detect the hemogram,the liver and renal function.5 cm sections of the terminal ileum were fixed in formalin for histopathological examination including the mean heisht of villous and the number of crypt.Small intestinal mucosal samples were homogenized for NO concentration analysis.Results In 10 Gy group,WBC and PLT decreased obviously,and the liver function was damaged.The length of villi and the number of crypt were significantly reduced in irradiated groups compared with control group,especially in 10 Gy group.Intestinal NO concentration decreased in 10 Gy group in 3 d.Conclusions To establish the mouse model of irradiated enteritis single 6 Gy60Co abdorminal irradiation is optimum.