CAJ | 학술논문

目的测定广州市2009年Ⅰ型登革病毒的E基因序列并进行分析,探讨其来源及基因型.方法收集广州市2009年登革热患者急性期血清19份,用C6/36细胞培养分离登革病毒,RT-PCR法扩增全长E基因,测定序列并绘制系统发生树,结合流行病学资料进行信息学分析.结果 19份标本中分离到4株Ⅰ型登革病毒株,测序获得其E基因序列.分析发现4株病毒来自两个不同的亚型,09/GZ/9104和09/GZ/9236两株Ⅰ型登革病毒核苷酸序列相同,属于美洲/非洲型;09/GZ/11534和09/GZ/11562两株Ⅰ型登革病毒序列同源性较高,属于亚洲型.结论广州市2009年Ⅰ型登革病毒属输入性,分属两个基因型,推测可能来源于不同的传染源.
목적 측정엄주시2009년Ⅰ형등혁병독적E기인서렬병진행분석,탐토기래원급기인형.방법 수집엄주시2009년등혁열환자급성기혈청19빈,용C6/36세포배양분리등혁병독,RT-PCR법확증전장E기인,측정서렬병회제계통발생수,결합류행병학자료진행신식학분석.결과 19빈표본중분리도4주Ⅰ형등혁병독주,측서획득기E기인서렬.분석발현4주병독래자량개불동적아형,09/GZ/9104화09/GZ/9236량주Ⅰ형등혁병독핵감산서렬상동,속우미주/비주형;09/GZ/11534화09/GZ/11562량주Ⅰ형등혁병독서렬동원성교고,속우아주형.결론 엄주시2009년Ⅰ형등혁병독속수입성,분속량개기인형,추측가능래원우불동적전염원.
Objective To sequence and analyze the envelope (E) gene of type Ⅰ dengue virus isolated from Guangzhou in 2009 for tracing the infection source. Methods The serum samples were collected from patients diagnosed with dengue fever in Guangzhou area during 2009. Dengue virus was isolated and cultured in C6/36 cells.The whole length of E gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and then sequenced. The phylogenetic tree was drawn by neighbor-joining method. The bioinformatics analysis was performed by combining the phylogenetic information and the epidemiology data. Results Four strains of type Ⅰ dengue virus were isolated from 19 samples. E gene of these strains was amplified and sequenced. The phylogenetic analysis showed that 09/GZ/9104 strain and 09/GZ/9236 strain had identical nucleotide sequence and fell within the American/African group, 09/GZ/11534 stain and 09/GZ/11562 strain had similar sequence homology and fell within the Asian group. Conclusion The typeⅠdengue viruses in Guangzhou area in 2009 are imported, which belong to two genotypes and may come from two independent origins respectively.