中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2011年
12期
983-987
,共5页
常娟%江昌新%王婷%尹海波
常娟%江昌新%王婷%尹海波
상연%강창신%왕정%윤해파
格雷夫斯病%桥本甲状腺炎%角蛋白19%波形蛋白%血管内皮生长因子C%环氧化物酶2
格雷伕斯病%橋本甲狀腺炎%角蛋白19%波形蛋白%血管內皮生長因子C%環氧化物酶2
격뢰부사병%교본갑상선염%각단백19%파형단백%혈관내피생장인자C%배양화물매2
Graves' disease%Hashimoto's thyroiditis%Cytokeratin-19%Vimentin%Vascular endothelial grouth factor-C%Cyclooxygenase-2
目的 探讨细胞角蛋白19(CK-19)、波形蛋白(vimentin)、血管内皮生长因子C(VEGF-C)和环氧化物酶2(COX-2)表达在弥漫性毒性甲状腺肿(diffuse toxic goiter,Graves病,GD)和桥本甲状腺炎(HT)发生和发展中的作用.方法 采用免疫组织化学SP法检测57例Graves病和58例桥本甲状腺炎中CK-19、波形蛋白、VEGF-C、COX-2的表达.结果 CK-19、VEGF-C、COX-2表达于甲状腺滤泡上皮细胞的胞浆,波形蛋白在间质和滤泡上皮细胞的胞浆均有表达.HT中CK-19和VEGF-C表达阳性率(86.2％、96.6％)及阳性强度均高于GD(43.9％、56.1％,均P＜0.05),波形蛋白和COX-2表达的阳性率(100％、93.1％)近似于GD( 100％、91.2％),但COX-2的阳性强度明显高于GD(均P＜0.05).CK-19阳性率在GDⅢ型(81.3％)明显高于Ⅰ型(15.8％)和Ⅱ型(40.9％),HT的P型(100％)明显高于L型(66.7％).VEGF-C阳性率在GDⅢ型(87.5％)明显高于Ⅰ型(36.8％)和Ⅱ型(50.0％,均P＜0.05).结论 综合检测CK-19、波形蛋白、VEGF-C和COX-2四种免疫学指标,对探讨GD和HT发生、发展过程及预后评估具有一定的参考价值.
目的 探討細胞角蛋白19(CK-19)、波形蛋白(vimentin)、血管內皮生長因子C(VEGF-C)和環氧化物酶2(COX-2)錶達在瀰漫性毒性甲狀腺腫(diffuse toxic goiter,Graves病,GD)和橋本甲狀腺炎(HT)髮生和髮展中的作用.方法 採用免疫組織化學SP法檢測57例Graves病和58例橋本甲狀腺炎中CK-19、波形蛋白、VEGF-C、COX-2的錶達.結果 CK-19、VEGF-C、COX-2錶達于甲狀腺濾泡上皮細胞的胞漿,波形蛋白在間質和濾泡上皮細胞的胞漿均有錶達.HT中CK-19和VEGF-C錶達暘性率(86.2％、96.6％)及暘性彊度均高于GD(43.9％、56.1％,均P＜0.05),波形蛋白和COX-2錶達的暘性率(100％、93.1％)近似于GD( 100％、91.2％),但COX-2的暘性彊度明顯高于GD(均P＜0.05).CK-19暘性率在GDⅢ型(81.3％)明顯高于Ⅰ型(15.8％)和Ⅱ型(40.9％),HT的P型(100％)明顯高于L型(66.7％).VEGF-C暘性率在GDⅢ型(87.5％)明顯高于Ⅰ型(36.8％)和Ⅱ型(50.0％,均P＜0.05).結論 綜閤檢測CK-19、波形蛋白、VEGF-C和COX-2四種免疫學指標,對探討GD和HT髮生、髮展過程及預後評估具有一定的參攷價值.
목적 탐토세포각단백19(CK-19)、파형단백(vimentin)、혈관내피생장인자C(VEGF-C)화배양화물매2(COX-2)표체재미만성독성갑상선종(diffuse toxic goiter,Graves병,GD)화교본갑상선염(HT)발생화발전중적작용.방법 채용면역조직화학SP법검측57례Graves병화58례교본갑상선염중CK-19、파형단백、VEGF-C、COX-2적표체.결과 CK-19、VEGF-C、COX-2표체우갑상선려포상피세포적포장,파형단백재간질화려포상피세포적포장균유표체.HT중CK-19화VEGF-C표체양성솔(86.2％、96.6％)급양성강도균고우GD(43.9％、56.1％,균P＜0.05),파형단백화COX-2표체적양성솔(100％、93.1％)근사우GD( 100％、91.2％),단COX-2적양성강도명현고우GD(균P＜0.05).CK-19양성솔재GDⅢ형(81.3％)명현고우Ⅰ형(15.8％)화Ⅱ형(40.9％),HT적P형(100％)명현고우L형(66.7％).VEGF-C양성솔재GDⅢ형(87.5％)명현고우Ⅰ형(36.8％)화Ⅱ형(50.0％,균P＜0.05).결론 종합검측CK-19、파형단백、VEGF-C화COX-2사충면역학지표,대탐토GD화HT발생、발전과정급예후평고구유일정적삼고개치.
Objective To study the roles of cytokeratin-19 ( CK-19 ),vimentin,vascular endothelial growth factor-C( VEGF-C ),and cyclooxygenase-2 ( COX-2 ) played in the occurrence and development of Graves'disease(GD) and Hashimoto's thyroiditis(HT).Methods 57 cases of GD and 58 cases of HT were enrolled in our study.Immunohistochemistry using SP method was carried out for assessment of the expression of CK-19,vimentin,VEGF-C,and COX-2 in the thyroid tissues.Results CK-19,VEGF-C,and COX-2 were expressed in the cytoplasm of thyroid follicular epithelial cells.Vimentin was expressed both in the mesenchyma and in the cytoplasm of thyroid follicular epithelial cells.The positive rates and expression intensities of CK-19 and VEGF-C in HT ( 86.2％,96.6％ ) were significantly higher than those in GD ( 43.9％,56.1％,all P＜0.05 ).The expression intensities of vimentin and COX-2 in GD ( 100.0％,93.1％ ) were similar to those in HT ( 100.0％,91.2 ％ ),while the expression intensity of COX-2 in HT was significantly higher than that in GD( all P＜0.05 ).The positive rates of CK-1 9 were much higher in type Ⅲ ( 81.3％ ) of GD than in type Ⅰ ( 1 5.8％ ) and type Ⅱ ( 40.9％ ) of GD,and also higher in type P( 100％ ) of HT than in type L(66.7％ ) of HT.The positive rates of VEGF-C were much higher in type Ⅲ ( 87.5％ ) of GD than in type Ⅰ ( 36.8％ ) and type Ⅱ ( 50.0％,all P ＜ 0.05 ) of GD.Conclusion Immunohistochemical detection of the expression of CK-19,vimentin,VEGF-C,and COX-2 may carry clinical significance in revealing the occurrence and development as well as evaluating the prognosis of Graves'disease and Hashimoto's thyroiditis.
