中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2008年
2期
103-106
,共4页
方仁东%李霞%李静%沈鑫%武洁%桂晓虹%刘力%高谦%梅建
方仁東%李霞%李靜%瀋鑫%武潔%桂曉虹%劉力%高謙%梅建
방인동%리하%리정%침흠%무길%계효홍%류력%고겸%매건
分枝杆菌,结核%基因型%多重感染
分枝桿菌,結覈%基因型%多重感染
분지간균,결핵%기인형%다중감염
Mycobacterium tuberculosis%Genotype%Multiple infections
目的 鉴定结核分枝杆菌多重感染的存在.方法 从上海市疾病预防控制中心结核病参比实验室保存的菌株中,选择1999年至2004年在同一抗结核疗程中前后2次分离菌株的耐药表型改变的患者为研究对象.挑选并活化入选病例的首次分离株,分离培养单菌落.利用可变数目串联重复序列多态性分型(VNTR)方法,对每个分离株的30个单菌落进行基因型分析.结果 在22例入选病例中,2例患者的首次分离株中存在2种不同基因型的菌株.2种不同基因型菌株在单菌落中占的比例分别是24:6和29:1.其余20例患者分离株各自的30个单菌落的基因型相同.结论 上海地区结核病患者中存在结核分枝杆菌的多重感染.
目的 鑒定結覈分枝桿菌多重感染的存在.方法 從上海市疾病預防控製中心結覈病參比實驗室保存的菌株中,選擇1999年至2004年在同一抗結覈療程中前後2次分離菌株的耐藥錶型改變的患者為研究對象.挑選併活化入選病例的首次分離株,分離培養單菌落.利用可變數目串聯重複序列多態性分型(VNTR)方法,對每箇分離株的30箇單菌落進行基因型分析.結果 在22例入選病例中,2例患者的首次分離株中存在2種不同基因型的菌株.2種不同基因型菌株在單菌落中佔的比例分彆是24:6和29:1.其餘20例患者分離株各自的30箇單菌落的基因型相同.結論 上海地區結覈病患者中存在結覈分枝桿菌的多重感染.
목적 감정결핵분지간균다중감염적존재.방법 종상해시질병예방공제중심결핵병삼비실험실보존적균주중,선택1999년지2004년재동일항결핵료정중전후2차분리균주적내약표형개변적환자위연구대상.도선병활화입선병례적수차분리주,분리배양단균락.이용가변수목천련중복서렬다태성분형(VNTR)방법,대매개분리주적30개단균락진행기인형분석.결과 재22례입선병례중,2례환자적수차분리주중존재2충불동기인형적균주.2충불동기인형균주재단균락중점적비례분별시24:6화29:1.기여20례환자분리주각자적30개단균락적기인형상동.결론 상해지구결핵병환자중존재결핵분지간균적다중감염.
Objective To identify multiple infections of Mycobaeterium tuberculosis in tuberculosis patients.Methods From the clinical isolates of M.tuberculosis during 1999 to 2004 stored at Shanghai Municipal Center for Disease Control and Prevention.cases who had two isolates with different drug resistance patterns in the same treatment episode were selected.The first isolate from the selected patients was inoculated onto Middlebrook 7H11 agar plates.and 30 single colonies from each isolate were picked and genotyped by the 7 loci Variable Number Tandem Repeat(VNTR)method.Results Two out of 22 isolates had two different genotypes among their 30 single colonies,and the proportions of the two genotype colonies Conclusion Multiple infections of M.tuberculosis occurred in tuberculosis patients in Shanghai.
