中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2011年
12期
810-814
,共5页
刘恩彬%陈辉树%张培红%李占琦%孙琦%杨晴英%方立环%孙福军
劉恩彬%陳輝樹%張培紅%李佔琦%孫琦%楊晴英%方立環%孫福軍
류은빈%진휘수%장배홍%리점기%손기%양청영%방립배%손복군
白血病,淋巴细胞%杀伤细胞,天然%穿刺术%免疫表型分型
白血病,淋巴細胞%殺傷細胞,天然%穿刺術%免疫錶型分型
백혈병,림파세포%살상세포,천연%천자술%면역표형분형
Leukemia,lymphocytic%Killer cells,natural%Punctures%Immunophenotyping
目的 探讨侵袭性NK细胞白血病(ANKL)的临床病理学特点.方法 回顾性分析10例ANKL患者的临床病理档案资料,全部病例均行全血细胞计数以及外周血涂片、骨髓穿刺与骨髓活检标本的形态学观察.用流式细胞学(FCM)及免疫组织化学(EliVision法)进行免疫表型分析.聚合酶链反应(PCR)法检测T细胞受体(TCR)γ基因重排.结果 10例患者中,最常见的血液学异常为贫血(7例)与血小板减少(9例).6例外周血涂片可见大颗粒淋巴细胞.骨髓穿刺涂片示8例淋巴细胞比例增高(>20.0%).6例可见大颗粒淋巴细胞.骨髓活检切片示轻度浸润5例,中度浸润3例,重度浸润2例.骨髓切片中8例为间质型浸润,2例呈弥漫型浸润,4例可见噬血现象(吞噬成熟红细胞).免疫表型方面,FCM检测示全部病例为CD2+sCD3- CD4- CD56+CD57-.9例CD7、5例CD16、4例CD8和1例CD5阳性.8例行免疫组织化学相关抗原检测:cCD3 4例、CD566例、T细胞内抗原1(TIA-1)6例、颗粒酶B4例和穿孔素2例阳性.10例TCRγ基因重排检测均为胚系构型.结论 ANKL是一种NK细胞来源的高度侵袭性淋巴组织肿瘤,需进行全面的外周血与骨髓的形态学、免疫表型以及分子遗传学检测才能确诊,需注意与多种NK细胞与T细胞淋巴瘤鉴别.
目的 探討侵襲性NK細胞白血病(ANKL)的臨床病理學特點.方法 迴顧性分析10例ANKL患者的臨床病理檔案資料,全部病例均行全血細胞計數以及外週血塗片、骨髓穿刺與骨髓活檢標本的形態學觀察.用流式細胞學(FCM)及免疫組織化學(EliVision法)進行免疫錶型分析.聚閤酶鏈反應(PCR)法檢測T細胞受體(TCR)γ基因重排.結果 10例患者中,最常見的血液學異常為貧血(7例)與血小闆減少(9例).6例外週血塗片可見大顆粒淋巴細胞.骨髓穿刺塗片示8例淋巴細胞比例增高(>20.0%).6例可見大顆粒淋巴細胞.骨髓活檢切片示輕度浸潤5例,中度浸潤3例,重度浸潤2例.骨髓切片中8例為間質型浸潤,2例呈瀰漫型浸潤,4例可見噬血現象(吞噬成熟紅細胞).免疫錶型方麵,FCM檢測示全部病例為CD2+sCD3- CD4- CD56+CD57-.9例CD7、5例CD16、4例CD8和1例CD5暘性.8例行免疫組織化學相關抗原檢測:cCD3 4例、CD566例、T細胞內抗原1(TIA-1)6例、顆粒酶B4例和穿孔素2例暘性.10例TCRγ基因重排檢測均為胚繫構型.結論 ANKL是一種NK細胞來源的高度侵襲性淋巴組織腫瘤,需進行全麵的外週血與骨髓的形態學、免疫錶型以及分子遺傳學檢測纔能確診,需註意與多種NK細胞與T細胞淋巴瘤鑒彆.
목적 탐토침습성NK세포백혈병(ANKL)적림상병이학특점.방법 회고성분석10례ANKL환자적림상병리당안자료,전부병례균행전혈세포계수이급외주혈도편、골수천자여골수활검표본적형태학관찰.용류식세포학(FCM)급면역조직화학(EliVision법)진행면역표형분석.취합매련반응(PCR)법검측T세포수체(TCR)γ기인중배.결과 10례환자중,최상견적혈액학이상위빈혈(7례)여혈소판감소(9례).6예외주혈도편가견대과립림파세포.골수천자도편시8례림파세포비례증고(>20.0%).6례가견대과립림파세포.골수활검절편시경도침윤5례,중도침윤3례,중도침윤2례.골수절편중8례위간질형침윤,2례정미만형침윤,4례가견서혈현상(탄서성숙홍세포).면역표형방면,FCM검측시전부병례위CD2+sCD3- CD4- CD56+CD57-.9례CD7、5례CD16、4례CD8화1례CD5양성.8례행면역조직화학상관항원검측:cCD3 4례、CD566례、T세포내항원1(TIA-1)6례、과립매B4례화천공소2례양성.10례TCRγ기인중배검측균위배계구형.결론 ANKL시일충NK세포래원적고도침습성림파조직종류,수진행전면적외주혈여골수적형태학、면역표형이급분자유전학검측재능학진,수주의여다충NK세포여T세포림파류감별.
Objective To study the clinicopathologic features of aggressive natural killer cell leukemia (ANKL).Methods The clinical and pathologic features were analyzed in 10 patients with ANKL.The complete blood count,peripheral blood smears,bone marrow aspirates and bone marrow biopsies were studied. Immunophenotypic analysis was carried out by flow cytometry and immunohistochemistry.T-cell receptor (TCR) γ gene rearrangement was studied by PCR method.Results The most frequent hematologic abnormalities observed were anemia (7 cases ) and thrombocytopenia (9 cases).Large granular lymphocytes were found on peripheral blood smears of 6 patients.In bone marrow aspirates,lymphocytosis ( > 20.0% ) was demonstrated in 8 cases and large granular lymphocytes in 6 cases.Bone marrow biopsies revealed various degrees of neoplastic infiltration,as follows:mild (5 cases),moderate (3 cases) and severe (2 cases).The neoplastic cells were mainly interstitial in distribution in 8 cases and diffuse in 2 cases.Hemophagocytosis was observed in 4 cases.Flow cytometry showedCD2+ sCD3- CD4- CD56+ CD57- in all cases,CD7 + in9 cases,CD16+ in 5 cases,CD8 + in 4 cases and CD5 + in 1 case.lmmunohistochemistry performed in 8 cases showed the following results:cCD3 + in 4 cases,CD56 + in 6 cases,TIA-1 + in 6 cases,granzyme B + in 4 cases and perforin + in 2 cases.PCR study revealed germline TCRγ gene configuration in all cases.Conclusions ANKL is a highly aggressive NK cell-derived lymphoid neoplasm. Comprehensive morphologic,immunophenotypic and molecular analysis are essential in arriving at a correct diagnosis.ANKL needs to be distinguished from other types of NK-cell and T-cell lymphomas.