CAJ | 학술논문

目的观察辅助性T细胞17(Th17)免疫应答是否参与颅内动脉瘤形成过程,检测细胞因子白细胞介素17(IL-17)及其Th17免疫应答特异性标志物-特异转录因子孤束核受体(RORγt)在大鼠和人颅内动脉瘤壁中的表达.方法建立肾性高血压诱导的Sprague-Dawley(SD)大鼠颅内动脉瘤模型,获取8个SD大鼠颅内动脉瘤.另取15例人颅内动脉瘤组织标本,用免疫组织化学方法检测IL-17、RORγt在大鼠脑动脉瘤壁中的表达,以及人脑动脉瘤壁IL-17、RORγt、白细胞共同抗原(LCA)和CD4的表达.结果 SD大鼠颅内动脉瘤壁上IL-17和RORγt有明显表达,且IL-17和RORγt表达呈正相关(r=0.917,P＜0.01),而正常对照SD大鼠脑动脉血管壁上无IL-17和RORγt的表达.IL-17和RORrt在人颅内动脉瘤壁也明显表达且呈正相关(r=0.903,P＜0.01),而正常对照颞浅动脉壁上无IL-17和RORγt的表达.同时,在人脑动脉瘤壁中IL-17和RORγt阳性表达的部位LCA和CD4也呈阳性.结论在大鼠和人的颅内动脉瘤壁均检测到IL-17和RORγt表达,表明Th17应答可能参与了颅内动脉瘤的形成.
목적 관찰보조성T세포17(Th17)면역응답시부삼여로내동맥류형성과정,검측세포인자백세포개소17(IL-17)급기Th17면역응답특이성표지물-특이전록인자고속핵수체(RORγt)재대서화인로내동맥류벽중적표체.방법 건립신성고혈압유도적Sprague-Dawley(SD)대서로내동맥류모형,획취8개SD대서로내동맥류.령취15례인로내동맥류조직표본,용면역조직화학방법검측IL-17、RORγt재대서뇌동맥류벽중적표체,이급인뇌동맥류벽IL-17、RORγt、백세포공동항원(LCA)화CD4적표체.결과 SD대서로내동맥류벽상IL-17화RORγt유명현표체,차IL-17화RORγt표체정정상관(r=0.917,P＜0.01),이정상대조SD대서뇌동맥혈관벽상무IL-17화RORγt적표체.IL-17화RORrt재인로내동맥류벽야명현표체차정정상관(r=0.903,P＜0.01),이정상대조섭천동맥벽상무IL-17화RORγt적표체.동시,재인뇌동맥류벽중IL-17화RORγt양성표체적부위LCA화CD4야정양성.결론 재대서화인적로내동맥류벽균검측도IL-17화RORγt표체,표명Th17응답가능삼여료로내동맥류적형성.
Objective To detect the level of interleukin 17 (IL-17) and transcription factor of orphan nuclear hormone receptor-γt (RORγt) in the intracranial aneurysm of humans and rats, and confirm whether T helper 17 ( Th17 ) takes part in the formation of intracranial aneurysm. Methods The models of intracranial aneurysm induced by nephrotic hypertension in Sprague-Dawley (SD) rats were established.Eight aneurysmal changes were found in the junction of the anterior cerebral (ACA) and the olfactort artery (OA) of rats, and immunohistochemistry was used to detect the expression level of IL-17 and RORγt, as well as 15 patients with intracranial aneurysms. Results Both IL-17 and RORγt were obviously expressed in the brain of rats with intracranial aneurysms as compared with the opposite control group. The percentage of IL-17 positive cells was associated with the percentage of RORγt positive cells (r =0. 917 ,P ＜0.01 ).No intracranial aneurysm was induced in control group. Both IL-17 and RORγt in patients with intracranial aneurysms were obviously expressed, as well as LCA and CD4. The percentage of IL-17 positive cells was associated with the percentage of RORγt positive cells (r =0. 903,P ＜0. 01 ). There was no expression of IL-17 and RORγt in the superficial temporal artery of normal controls. Conclusion Obvious expression of IL-17 and RORγt in the intracranial aneurysm of humans and rats may imply that Th17 response involves in the formation of intracranial aneurysm.