CAJ | 학술논문

目的研究双氢青蒿素(DHA)抑制胶质瘤生长作用、机制及联合替莫唑胺(TMZ)的协同抗肿瘤作用.方法选用胶质瘤细胞株10个,MTT法检测DHA持续作用72 h后细胞株半数抑制浓度( IC50)；0.5 μg/ml浓度DHA联合梯度浓度TMZ作用于SKMG -4细胞株,MTT法检测IC50；MDC法荧光分析DHA作用后自噬泡形成；梯度浓度DHA作用SKMG -4,Western Blot法检测caspase -3、Beclin-1、LC3-B蛋白表达.结果 DHA抑制胶质瘤细胞生长IC50为(1.17±0.078) μg/ml ～ (23.568±0.796) μg/ml；在SKMG -4细胞株中,DHA实验组与空白对照相比,可见明显的自噬泡染色；Beclin-1及LC3 -B表达随DHA浓度增加而增加,而caspase -3表达无明显变化；DHA联合TMZ抑制SKMG-4生长,IC50值明显下降(P＜0.01).结论 DHA具有抗胶质瘤活性,诱导胶质瘤细胞自噬；DHA联合作用可提高TMZ的抗胶质瘤SKMG -4活性,机制可能为增强了TMZ自噬效能.
목적 연구쌍경청호소(DHA)억제효질류생장작용、궤제급연합체막서알(TMZ)적협동항종류작용.방법 선용효질류세포주10개,MTT법검측DHA지속작용72 h후세포주반수억제농도( IC50)；0.5 μg/ml농도DHA연합제도농도TMZ작용우SKMG -4세포주,MTT법검측IC50；MDC법형광분석DHA작용후자서포형성；제도농도DHA작용SKMG -4,Western Blot법검측caspase -3、Beclin-1、LC3-B단백표체.결과 DHA억제효질류세포생장IC50위(1.17±0.078) μg/ml ～ (23.568±0.796) μg/ml；재SKMG -4세포주중,DHA실험조여공백대조상비,가견명현적자서포염색；Beclin-1급LC3 -B표체수DHA농도증가이증가,이caspase -3표체무명현변화；DHA연합TMZ억제SKMG-4생장,IC50치명현하강(P＜0.01).결론 DHA구유항효질류활성,유도효질류세포자서；DHA연합작용가제고TMZ적항효질류SKMG -4활성,궤제가능위증강료TMZ자서효능.
Objective To investigate the anti -glioma efficacy of dihydroartemisinin (DHA) and combined with temozolomide (TMZ) on human glioma cell line in vitro.Methods The growth inhibition of DHA on 10 glioma cell lines induced by DHA treatment for 72 h was analyzed by MTT method.TMZ combined with 0.5 μg/ml DHA,and the IC5o value was measured in SKMG -4 cells.The autofluorescent drug monodansylcadaverine (MDC) was used to mark autophagicvacuoles.The autophagy related protein LC3 - B and Beclin - 1,and the apoptosis protein caspase - 3 were analyzed by western blot (WB).Results The IC50 of DHA was different in ten glioma cell lines [ from ( 1.17 ± 0.078 ) μg/ml to (23.568±0.796) μg/ml].In SKMG -4 cells,the autophagicvacuoles were detected in the DHA group,the IC50 of TMZ had significant difference with 0.5 μg/ml DHA contrast to the control group ( P ＜ 0.01 ),and the levels of LC3 - B and Beclin - 1 protein were increased gradually with the increase of DHA concentration,and caspase -3 protein has no difference.Conclusion DHA can inhibit proliferation of glioma cell lines and increase the efficacy of TMZ,and the autophagy may be the mechanism.