CAJ | 학술논문

目的检测人类精氨酸酶1(ARG1)在肝细胞癌(HCC)中的表达,分析其与患者临床病理特征的关系.方法利用高通量组织芯片技术和免疫组织化学方法评价167例HCC及癌旁肝组织中ARG1蛋白的表达情况,采用x2检验和Spearman秩相关分析ARG1蛋白表达与各临床病理特征间的关系.应用实时荧光定量PCR方法检测68例HCC及其癌旁肝组织中的ARG1 mRNA表达水平.结果 HCC中ARG1蛋白表达水平为3.540±3.702,明显低于癌旁肝组织(10.290±2.303；t=-22.421,P=0.000).ARG1表达与HCC分化程度、组织学分级、微血管侵犯、术前甲胎蛋白水平、术后复发有关(P值均＜0.05).68例HCC组织中ARG1 mRNA表达水平为0.0997(0.213),低于癌旁肝组织0.563(0.459)；u=-6.544,P=0.000].结论 ARG1在HCC中低表达,其可能参与HCC发生发展.检测ARG1表达可能有助于HCC辅助诊断、评估分化程度及预后判断.
목적 검측인류정안산매1(ARG1)재간세포암(HCC)중적표체,분석기여환자림상병리특정적관계.방법 이용고통량조직심편기술화면역조직화학방법평개167례HCC급암방간조직중ARG1단백적표체정황,채용x2검험화Spearman질상관분석ARG1단백표체여각림상병리특정간적관계.응용실시형광정량PCR방법검측68례HCC급기암방간조직중적ARG1 mRNA표체수평.결과 HCC중ARG1단백표체수평위3.540±3.702,명현저우암방간조직(10.290±2.303；t=-22.421,P=0.000).ARG1표체여HCC분화정도、조직학분급、미혈관침범、술전갑태단백수평、술후복발유관(P치균＜0.05).68례HCC조직중ARG1 mRNA표체수평위0.0997(0.213),저우암방간조직0.563(0.459)；u=-6.544,P=0.000].결론 ARG1재HCC중저표체,기가능삼여HCC발생발전.검측ARG1표체가능유조우HCC보조진단、평고분화정도급예후판단.
Objective To investigate the expression of arginase Ⅰ(ARG1)in hepatocellular carcinoma(HCC)and to analyze its correlation with clinicopathological features.Methods The expression of ARG1 at protein level in 167 samples of HCC and corresponding adjacent liver tissue was detected with high-throughput tissue microarray technique and immunohistochemistry.The correlation between ARG1 expression and clinicopathological features was analyzed with x2 test and Spearman rank correlation analysis.The expression of ARG1 at mRNA level in 68 samples of HCC and corresponding adjacent liver tissue was determined by real-time polymerase chain reaction(real-time PCR).Results The expression of ARG1 at protein level in HCC(3.540±3.702)was significantly lower than that of the corresponding adjacent liver tissues(10.290 ± 2.303)(t=-22.421,P=0.000).The ARG1 expression was correlated with differentiation degree of HCC,histological grade,vascular invasion,preoperative level of α-fetoprotein(AFP)and recurrence after operation(all P＜0.05).The ARG1 expression at mRNA level in 68 HCC tissue[0.0997(0.213)]was lower than that of the corresponding adjacent liver tissues[0.563(0.459)],and the difference was statistical significant(u=-6.544,P=0.000).Conclusion Low expression of ARG1 in HCC may take part inHCC genesis and development.Detecting the expression of ARG1 may be helpful in HCC diagnosis,differentiation degree and prognosis assessment.