CAJ | 학술논문

目的制备125I-103Pd复合型放射性粒子,并探讨其对肺癌的杀伤效果.方法利用化学镀层方法制备125I-103Pd放射性粒子.体外培养肺腺癌GLC-82细胞及大细胞癌H460细胞,将125I-103Pd.125I、103Pd及空白粒子置于细胞培养皿中照射48 h,观察粒子对肿瘤细胞的杀伤效应.40只BALB/c-nu小鼠随机平分为2组,分别构建GLC-82及H460的移植瘤模型,每种模型荷瘤鼠随机分成_4组,每组5只.将125I-103pd、125I、103Pd及空白粒子分别植入裸鼠的移植瘤内,每5天定期测量移植瘤大小及裸鼠体重变化,观察61 d.结果成功制备125I-103Pd粒子.粒子照射细胞48 h后,125I、103Pd及125I-103Pd粒子周围约1个粒子直径范围内的GLC-82细胞明显肿胀、变形,生长抑制;125I粒子周围H460细胞牛长无明显改变,而103Pd及125I-103Pd粒子周围约1个粒子直径内H460细胞生长稀少.体内实验小鼠无死亡,H460移植瘤较GLC-82移植瘤生长快,放射性粒子对两种移植瘤均有抑制,抑瘤效果:125I-103pd粒子>103Pd粒子≈125I粒子>空白粒子;同种放射性粒子对GLC-82移植瘤的抑制效果较H460移植瘤好.结论 125I-103Pd复合型放射性粒子制备工艺可行,抑瘤效果较好,具有较好的应用前景.
목적 제비125I-103Pd복합형방사성입자,병탐토기대폐암적살상효과.방법 이용화학도층방법제비125I-103Pd방사성입자.체외배양폐선암GLC-82세포급대세포암H460세포,장125I-103Pd.125I、103Pd급공백입자치우세포배양명중조사48 h,관찰입자대종류세포적살상효응.40지BALB/c-nu소서수궤평분위2조,분별구건GLC-82급H460적이식류모형,매충모형하류서수궤분성_4조,매조5지.장125I-103pd、125I、103Pd급공백입자분별식입라서적이식류내,매5천정기측량이식류대소급라서체중변화,관찰61 d.결과 성공제비125I-103Pd입자.입자조사세포48 h후,125I、103Pd급125I-103Pd입자주위약1개입자직경범위내적GLC-82세포명현종창、변형,생장억제;125I입자주위H460세포우장무명현개변,이103Pd급125I-103Pd입자주위약1개입자직경내H460세포생장희소.체내실험소서무사망,H460이식류교GLC-82이식류생장쾌,방사성입자대량충이식류균유억제,억류효과:125I-103pd입자>103Pd입자≈125I입자>공백입자;동충방사성입자대GLC-82이식류적억제효과교H460이식류호.결론 125I-103Pd복합형방사성입자제비공예가행,억류효과교호,구유교호적응용전경.
Objective To prepared 125I-103Pd hybrid radioactive seeds and to explore their therapeutic effect on pulmonary carcinomas.nethods The 125I-103Pd hybrid radioactive seeds were prepared by a chemical method of step-by-step coat plating.Pulmonary adenocarcinoma of GLC-82 cells and pulmonary large cell carcinoma of H460 cells were cultured in vitro and then were exposed directly to125I,103pd and125I103pd seeds for 48 hours to observe the killing effects of radiation.GLC-82 and H460 tumor models were established and 20 mice chosen randomly for each model.For each tumor model.there were 4 groups(n=5 each).Then 125I-103pd,125I,103Pd and nonradioactive seeds were implanted into the tumors.Tumor sizes and weights of mice were measured and recorded every 5 days for a 2-month observation.Resuits The125I-103Pd hybrid radioactive seeds were prepared successfully.After a 48-hour radiation from radioactive seeds, the GLC-82 cells within one particulate around 125I,103Pd or1 25I_103Pd seeds were inhibited so as to become swollen and transfiguring.The H460 cells around125I seeds showed no obvious abnormality while those within one Darticalate around 103Pd or125I-103pd seeds were much fewer. No mouse died during the observation period.The radioactive seeds could inhibit the tumors.The radiotherapeutie effects were similar in two tumor modes:125I_103Pd seeds>103Pd seeds≈125I seeds>non-radioactive seeds.H460 tumors grew much faster than GLC-82 tumors.Meanwhile the seeds with the same nuclide were much more effective for GLC-82 tumors than for H460 tumors.Conclusion TheI_l03pd hybrid radioactive seeds are clinically applieable due to their effective inhibitions of tumor growth.