中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
2期
140-146
,共7页
雷娜%端家忠%黄平%曾照丽%李丽%王晨%邢辉%邵一鸣%张晓曦%杨贵波
雷娜%耑傢忠%黃平%曾照麗%李麗%王晨%邢輝%邵一鳴%張曉晞%楊貴波
뢰나%단가충%황평%증조려%리려%왕신%형휘%소일명%장효희%양귀파
吲哚胺 2,3 双加氧酶%TaqMan 探针实时荧光定量 RT-PCR%HIV-1%TLR
吲哚胺 2,3 雙加氧酶%TaqMan 探針實時熒光定量 RT-PCR%HIV-1%TLR
신타알 2,3 쌍가양매%TaqMan 탐침실시형광정량 RT-PCR%HIV-1%TLR
Indoleamine 2,3-dioxygenase%TaqMan probe real-time RT-PCR%HIV-1%TLR
目的 定量分析HIV-1感染者及高危人群外周血单个核细胞(PBMC)和人源肿瘤细胞在Toll样受体(TLR)配体刺激前后吲哚胺2,3双加氧酶(indoleamine 2,3-dioxygenase,IDO)的表达水平.方法 建立人IDO mRNA的TaqMan探针实时荧光定量RT-PCR检测方法;检测HIV-1阳性个体和高危人群PBMC中IDO mRNA水平;检测TLR4、TLR7/8、TLR9配体刺激前后人黏膜(T84、Caco2、Hela)和白细胞(THP-1、MT-4)来源肿瘤细胞中IDO mRNA的水平.结果 HIV-1阳性个体PBMC中IDO mRNA水平较高危人群高(103.42拷贝IDO mRNA/106拷贝GAPDH mRNA);但也有部分高危人群个体的PBMC含有较高水平的IDO mRNA;肿瘤细胞在TLR配体刺激后IDO mRNA的水平可以显著升高,因细胞系和TLR配体种类不同而异.结论 本研究表明IDO可能在我国HIV-1感染者体内病毒免疫逃逸和肿瘤发生中起作用,值得深入研究.
目的 定量分析HIV-1感染者及高危人群外週血單箇覈細胞(PBMC)和人源腫瘤細胞在Toll樣受體(TLR)配體刺激前後吲哚胺2,3雙加氧酶(indoleamine 2,3-dioxygenase,IDO)的錶達水平.方法 建立人IDO mRNA的TaqMan探針實時熒光定量RT-PCR檢測方法;檢測HIV-1暘性箇體和高危人群PBMC中IDO mRNA水平;檢測TLR4、TLR7/8、TLR9配體刺激前後人黏膜(T84、Caco2、Hela)和白細胞(THP-1、MT-4)來源腫瘤細胞中IDO mRNA的水平.結果 HIV-1暘性箇體PBMC中IDO mRNA水平較高危人群高(103.42拷貝IDO mRNA/106拷貝GAPDH mRNA);但也有部分高危人群箇體的PBMC含有較高水平的IDO mRNA;腫瘤細胞在TLR配體刺激後IDO mRNA的水平可以顯著升高,因細胞繫和TLR配體種類不同而異.結論 本研究錶明IDO可能在我國HIV-1感染者體內病毒免疫逃逸和腫瘤髮生中起作用,值得深入研究.
목적 정량분석HIV-1감염자급고위인군외주혈단개핵세포(PBMC)화인원종류세포재Toll양수체(TLR)배체자격전후신타알2,3쌍가양매(indoleamine 2,3-dioxygenase,IDO)적표체수평.방법 건립인IDO mRNA적TaqMan탐침실시형광정량RT-PCR검측방법;검측HIV-1양성개체화고위인군PBMC중IDO mRNA수평;검측TLR4、TLR7/8、TLR9배체자격전후인점막(T84、Caco2、Hela)화백세포(THP-1、MT-4)래원종류세포중IDO mRNA적수평.결과 HIV-1양성개체PBMC중IDO mRNA수평교고위인군고(103.42고패IDO mRNA/106고패GAPDH mRNA);단야유부분고위인군개체적PBMC함유교고수평적IDO mRNA;종류세포재TLR배체자격후IDO mRNA적수평가이현저승고,인세포계화TLR배체충류불동이이.결론 본연구표명IDO가능재아국HIV-1감염자체내병독면역도일화종류발생중기작용,치득심입연구.
Objective To compare the levels of indoleamine 2,3-dioxygenase (IDO) in the peripheral blood mononuclear cells(PBMCs) from HIV-1 infected and HIV-1 negative individuals and in human tumor cells in the presence or absence of TLR ligand stimulation.Methods TaqMan probe real-time RT-PCR method for human IDO mRNA was established; IDO mRNA levels in the PBMCs from HIV-1+ and HIV-1-individuals were tested; IDO mnRNA levels in mucosal origin(T84,Caco-2,Hela) and leukocyte origin(THP-1,MT-4) tumor cells before and after exposure to agonists for TLR4,TLR7/8 and TLR9 were examined.Results It was found that a high level of IDO mRNA could be found in HIV-1+ individuals( 103.42 copy IDO mRNA/106 copy GAPDH mRNA) ; however,some high risk HIV-1-individuals may have also a high level of IDO mRNA.Some of the tumor cells could express higher level of IDO mRNA after exposure to TLR agonist.Conclusion This study indicated a role for IDO in the viral persistence and tumor formation in HIV/AIDS and further studies were warranted.
