CAJ | 학술논문

目的初步分析新发现的质荷比(M/Z)15 000+H～16 800+H范围内蛋白质组指纹表达与焦虑症的相关性.方法 2004年4月至2006年12月,对221例肿瘤患者进行了观察,全部采用焦虑自评量表(SAS)进行评估,根据评分标准分为焦虑症组和无焦虑组,应用蛋白质飞行时间质谱技术(SELDI-TOF-MS)检测患者血清,获得指纹图进行分析.结果采用SAS评估221例患者,其中焦虑症患者49例,无焦虑患者172例,在焦虑症组患者的血清中捕获到M/Z为15 000+H～15 380+H和15 700+H～16 800+H的质谱峰簇,与SAS评估的符合率为75.51%,其中,M/Z为15 700+H～16 800+H出现频度最高,其次是M/Z为15 000+H～15 380+H和15 700+H～16 800+H的双峰簇指纹,M/Z为15 000+H～15 380+H峰簇指纹出现频度最少.在SAS评估为无焦虑的患者血清中也捕获到上述指纹图谱,二者比较除丰度有差异外,其质荷比是一致的.结论 M/Z为15 000+H～16 800+H处的峰簇为焦虑症相关蛋白质指纹.
목적 초보분석신발현적질하비(M/Z)15 000+H～16 800+H범위내단백질조지문표체여초필증적상관성.방법 2004년4월지2006년12월,대221례종류환자진행료관찰,전부채용초필자평량표(SAS)진행평고,근거평분표준분위초필증조화무초필조,응용단백질비행시간질보기술(SELDI-TOF-MS)검측환자혈청,획득지문도진행분석.결과 채용SAS평고221례환자,기중초필증환자49례,무초필환자172례,재초필증조환자적혈청중포획도M/Z위15 000+H～15 380+H화15 700+H～16 800+H적질보봉족,여SAS평고적부합솔위75.51%,기중,M/Z위15 700+H～16 800+H출현빈도최고,기차시M/Z위15 000+H～15 380+H화15 700+H～16 800+H적쌍봉족지문,M/Z위15 000+H～15 380+H봉족지문출현빈도최소.재SAS평고위무초필적환자혈청중야포획도상술지문도보,이자비교제봉도유차이외,기질하비시일치적.결론 M/Z위15 000+H～16 800+H처적봉족위초필증상관단백질지문.
Objective A preliminary study on a new discovered proteomic fingerprint correlated with anxiety disorders, which M/Z range from 15000 to 16800. Methods 221 cases of neoplasm patients from April of 2004 to now were divided into anxious group and non-anxious group according to their scores using self-rating anxiety scale (SAS), and all the patients' sera were detected utilizing SELDI-TOF-MS. The data were analyzed with supported softwares. Results Compared the mass spectra of anxious group(n=49) and non-anxious group (n=172), 2 clusters were captured which M/Z range from 15 000 to 15 380 and 15 700 to 16 800 respectively. The coincidence with SAS was 75.51%. The M/Z range from 15 700 to 16 800 was the most frequent, then the second range from 15 000 to 15 380 and 15 700 to 16 800 which was doublet cluster, the least range from 15 000 to 15 380. The same fingerprints were captured in non-anxious group and their intensity was different. Conclusion The M/Z range from 15 000 to 16 800 was protein fingerprint correlated with anxiety disorders.