肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
11期
759-761
,共3页
陈钢%王春利%郭石平%苏文%乔丽娟%麦羡霞%马捷%胡成广%张沛刚
陳鋼%王春利%郭石平%囌文%喬麗娟%麥羨霞%馬捷%鬍成廣%張沛剛
진강%왕춘리%곽석평%소문%교려연%맥이하%마첩%호성엄%장패강
肺肿瘤%受体%神经激肽2%基因%MHCⅠ类%免疫逃逸%肿瘤
肺腫瘤%受體%神經激肽2%基因%MHCⅠ類%免疫逃逸%腫瘤
폐종류%수체%신경격태2%기인%MHCⅠ류%면역도일%종류
Lung neoplasms%Receptors%neurokinin-2%Genes%MHC class I%Immune escape%tumor
目的 探讨肺癌患者自然杀伤细胞活性受体(NKG2D)与可溶性MICA(sMICA)的表达及其二者的相关性.方法 选取30例肺癌患者作为试验组,30例健康志愿者作为对照组,采用流式细胞术(FCM)与酶联免疫吸附(ELISA)法分别测定两组NKG2D与sMICA分子的表达.结果 肺癌患者和健康人群NKG2D分子表达分别为(81.56±8.78)%、(85.63±6.62)%,二者差异有统计学意义(P<0.05).肺癌患者和健康对照血清中sMICA分子表达分别为(354.13±80.575)pg/ml、(216.53±48.175)pg/ml,二者差异有统计学意义(P<0.01).NKG2D和血清中sMICA分子两个变量之间具有明显相关性(r=-0.349,P=0.006),呈负相关关系.结论 NKG2D的表达和血清中sMICA的水平可能成为预测肺癌患者免疫功能状态及肿瘤恶性转移的指标之一.sMICA表达及对NKG2D的负调节机制可能是导致肿瘤细胞发生免疫逃逸的原因之一.该机制可能会为肿瘤生物免疫治疗提供患者适应证指标.
目的 探討肺癌患者自然殺傷細胞活性受體(NKG2D)與可溶性MICA(sMICA)的錶達及其二者的相關性.方法 選取30例肺癌患者作為試驗組,30例健康誌願者作為對照組,採用流式細胞術(FCM)與酶聯免疫吸附(ELISA)法分彆測定兩組NKG2D與sMICA分子的錶達.結果 肺癌患者和健康人群NKG2D分子錶達分彆為(81.56±8.78)%、(85.63±6.62)%,二者差異有統計學意義(P<0.05).肺癌患者和健康對照血清中sMICA分子錶達分彆為(354.13±80.575)pg/ml、(216.53±48.175)pg/ml,二者差異有統計學意義(P<0.01).NKG2D和血清中sMICA分子兩箇變量之間具有明顯相關性(r=-0.349,P=0.006),呈負相關關繫.結論 NKG2D的錶達和血清中sMICA的水平可能成為預測肺癌患者免疫功能狀態及腫瘤噁性轉移的指標之一.sMICA錶達及對NKG2D的負調節機製可能是導緻腫瘤細胞髮生免疫逃逸的原因之一.該機製可能會為腫瘤生物免疫治療提供患者適應證指標.
목적 탐토폐암환자자연살상세포활성수체(NKG2D)여가용성MICA(sMICA)적표체급기이자적상관성.방법 선취30례폐암환자작위시험조,30례건강지원자작위대조조,채용류식세포술(FCM)여매련면역흡부(ELISA)법분별측정량조NKG2D여sMICA분자적표체.결과 폐암환자화건강인군NKG2D분자표체분별위(81.56±8.78)%、(85.63±6.62)%,이자차이유통계학의의(P<0.05).폐암환자화건강대조혈청중sMICA분자표체분별위(354.13±80.575)pg/ml、(216.53±48.175)pg/ml,이자차이유통계학의의(P<0.01).NKG2D화혈청중sMICA분자량개변량지간구유명현상관성(r=-0.349,P=0.006),정부상관관계.결론 NKG2D적표체화혈청중sMICA적수평가능성위예측폐암환자면역공능상태급종류악성전이적지표지일.sMICA표체급대NKG2D적부조절궤제가능시도치종류세포발생면역도일적원인지일.해궤제가능회위종류생물면역치료제공환자괄응증지표.
Objective To investigate the expression and correlation of NKG2D and sMICA in lung cancer patients. Methods By collecting 30 lung cancer patients as the test group,and taking 30 healthy volunteers as the contrast group, the expression of NKG2D and sMICA in the two groups were examined separately by FACS and ELISA method. Results The expressions of NKG2D in the two groups were (81.56±8.78) %, (85.63±6.62) %. The lung cancer patients were high remarkable. There was a significant difference between the two groups (P <0.05). The expression of sMICA in the two groups were (354.13 ±80.575) pg/ml,(216.53±48.175) pg/ml. The lung cancer patients were low remarkable. There was a significant difference between the two groups (P <0.01). There was a significant relation between the two groups (r =-0.349, P =0.006). Conclusion The expression of NKG2D and sMICA may provid one of the immune targets for diagnosing that can forecast the immune state and malignant metastasis of the lung cancer patients. The significant relation between NKG2D and sMICA may take on main role in the immune escaping of tumor. It may provide the suitable target of the patients for tumor organisms and immune treatment.
