中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
4期
233-235
,共3页
李静%程留芳%李贞%丛玉文
李靜%程留芳%李貞%叢玉文
리정%정류방%리정%총옥문
肝硬化%肠黏膜%淋巴细胞
肝硬化%腸黏膜%淋巴細胞
간경화%장점막%림파세포
Liver cirrhosis%Intestinal mucosa%Lymphocytes
目的 研究肝硬化大鼠模型肠上皮内T淋巴细胞的表达,了解肝硬化肠黏膜免疫屏障功能的改变.方法 采用皮下注射40%四氯化碳诱导肝硬化模型,分离大鼠肠上皮内淋巴细胞,分别标记小鼠抗大鼠CD3、CD4、CD8单克隆抗体,流式细胞仪检测T淋巴细胞表达情况.结果 正常对照组和肝硬化模型组分离肠上皮内淋巴细胞数量分别为(2.3±0.5)×105 /cm、(3.4±1.1)×105 /cm,模型组高于对照组(P＜0.05);两组CD3+ T淋巴细胞比例分别为(80±6)%、(76±8)%,模型组有下降趋势,但两组间差异无统计学意义(P＞0.05);两组中CD4+ CD8+亚群细胞分别是(3.7±1.8)%、(6.9±3.3)%,模型组比例多于对照组(P＜0.05);CD4+ CD8-亚群细胞分别是(6.9±3.0)%、(4.4±1.4)%,模型组比例少于对照组(P＜0.05).结论 肝硬化大鼠模型肠上皮内T淋巴细胞表达的变化反映了肝硬化肠黏膜免疫屏障功能的改变.
目的 研究肝硬化大鼠模型腸上皮內T淋巴細胞的錶達,瞭解肝硬化腸黏膜免疫屏障功能的改變.方法 採用皮下註射40%四氯化碳誘導肝硬化模型,分離大鼠腸上皮內淋巴細胞,分彆標記小鼠抗大鼠CD3、CD4、CD8單剋隆抗體,流式細胞儀檢測T淋巴細胞錶達情況.結果 正常對照組和肝硬化模型組分離腸上皮內淋巴細胞數量分彆為(2.3±0.5)×105 /cm、(3.4±1.1)×105 /cm,模型組高于對照組(P＜0.05);兩組CD3+ T淋巴細胞比例分彆為(80±6)%、(76±8)%,模型組有下降趨勢,但兩組間差異無統計學意義(P＞0.05);兩組中CD4+ CD8+亞群細胞分彆是(3.7±1.8)%、(6.9±3.3)%,模型組比例多于對照組(P＜0.05);CD4+ CD8-亞群細胞分彆是(6.9±3.0)%、(4.4±1.4)%,模型組比例少于對照組(P＜0.05).結論 肝硬化大鼠模型腸上皮內T淋巴細胞錶達的變化反映瞭肝硬化腸黏膜免疫屏障功能的改變.
목적 연구간경화대서모형장상피내T림파세포적표체,료해간경화장점막면역병장공능적개변.방법 채용피하주사40%사록화탄유도간경화모형,분리대서장상피내림파세포,분별표기소서항대서CD3、CD4、CD8단극륭항체,류식세포의검측T림파세포표체정황.결과 정상대조조화간경화모형조분리장상피내림파세포수량분별위(2.3±0.5)×105 /cm、(3.4±1.1)×105 /cm,모형조고우대조조(P＜0.05);량조CD3+ T림파세포비례분별위(80±6)%、(76±8)%,모형조유하강추세,단량조간차이무통계학의의(P＞0.05);량조중CD4+ CD8+아군세포분별시(3.7±1.8)%、(6.9±3.3)%,모형조비례다우대조조(P＜0.05);CD4+ CD8-아군세포분별시(6.9±3.0)%、(4.4±1.4)%,모형조비례소우대조조(P＜0.05).결론 간경화대서모형장상피내T림파세포표체적변화반영료간경화장점막면역병장공능적개변.
Objective To investigate the phenotypes of T lymphocytes in the intestinal epithelium in cirrhosis.Methods Forty Wistar rats were randomly divided into 2 groups:cirrhosis group(n=25)undergoing subcutaneous injection of 40% carbon tetrachloride twice a week for 10-12 weeks to establish cirrhosis models,and control group (n=15).Twelve weeks later the rats were killed with their levers taken out.The T lymphocytes in the intestinal epithelium were isolated and labeled with mouse anti-rat CD3,CD4 and CD8 monoclonal antibody, Flow cytometry was performed. Results The number of intestinal intraepithelial lymphocytes of the cirrhosis group was(3.4±1.1)×105 /cm,significantly higher than that of the control group [(2.3±0.5)×105 /cm,P＜0.05].The proportion of CD3+ cells of the cirrhosis group were(76±8)%,not significantly different from that of the control group [(80±6)%,P＞0.05].There were no significant different between two group(P＞0.05).The proportion of CD4+ CD8+ subpopulation of the cirrhosis group was(6.9±3.3)%,significantly higher than that of the control group[(3.7±1.8)%,P＜0.05].The proportion of CD4+ CD8- subpopulation of the cirrhosis group was(6.9±3.0)%,significantly higher than that of the control group [(4.4±1.4)%,P＜0.05].Conclusion There were alterations of the immune barrier function in cirrhosis.The increase of the proportion of CD4+ CD8+ subpopulation may be associated with the increases of the number of intestinal bacteria.
