中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2009年
6期
380-384
,共5页
周吕%袁勃%王礼建%侯晓菲
週呂%袁勃%王禮建%侯曉菲
주려%원발%왕례건%후효비
结肠%动力%多潘立酮%莫沙必利%西沙必利
結腸%動力%多潘立酮%莫沙必利%西沙必利
결장%동력%다반립동%막사필리%서사필리
Colon%Motility%Domperidone%Mosapride%Cisapride
目的 观察多潘立酮对结肠的促动力作用,并与莫沙必利及西沙必利比较.方法 ①在体实验:将40只大鼠分为对照组、多潘立酮组、莫沙必利组和西沙必利组,每组10只.在各组大鼠近端结肠和远端结肠埋植应力传感器,记录清醒大鼠结肠运动.②离体实验:在恒温灌流肌槽中,采用张力传感器测定多巴胺和多巴胺+多潘立酮对大鼠离体结肠肌条的收缩活动.结果 在体实验:①清醒大鼠在消化间期的静息状态下,结肠呈现节律性相位收缩活动.②多潘立酮可明显增加结肠的收缩活动,使近端结肠和远端结肠平均振幅分别比对照组增加84.61%±7.26%和76.37%±8.47%,呈剂量-效应关系.同等剂量的莫沙必利对近端结肠和远端结肠平均振幅分别比对照组增加50.32%±8.16%和45.13%±7.16%.莫沙必利对结肠的促动力作用明显低于多潘立酮.而同等剂量的西沙必利对近端结肠和远端结肠平均振幅分别比对照组增加92.55%±8.37%和81.27%±9.95%,其促动力作用与多潘立酮相同.离体实验:①灌流多巴胺(40 mg/ml)可明显抑制离体结肠肌条收缩活动,较Krebs-Ringer液对照组减少91.56%±10.24%.②多潘立酮可阻断多巴胺对离体结肠肌条的舒张作用.结论 多潘立酮可明显增强结肠动力,其作用明显优于莫沙必利,并与西沙必利作用相同.多潘立酮增强结肠动力作用是通过抗多巴胺作用实现.
目的 觀察多潘立酮對結腸的促動力作用,併與莫沙必利及西沙必利比較.方法 ①在體實驗:將40隻大鼠分為對照組、多潘立酮組、莫沙必利組和西沙必利組,每組10隻.在各組大鼠近耑結腸和遠耑結腸埋植應力傳感器,記錄清醒大鼠結腸運動.②離體實驗:在恆溫灌流肌槽中,採用張力傳感器測定多巴胺和多巴胺+多潘立酮對大鼠離體結腸肌條的收縮活動.結果 在體實驗:①清醒大鼠在消化間期的靜息狀態下,結腸呈現節律性相位收縮活動.②多潘立酮可明顯增加結腸的收縮活動,使近耑結腸和遠耑結腸平均振幅分彆比對照組增加84.61%±7.26%和76.37%±8.47%,呈劑量-效應關繫.同等劑量的莫沙必利對近耑結腸和遠耑結腸平均振幅分彆比對照組增加50.32%±8.16%和45.13%±7.16%.莫沙必利對結腸的促動力作用明顯低于多潘立酮.而同等劑量的西沙必利對近耑結腸和遠耑結腸平均振幅分彆比對照組增加92.55%±8.37%和81.27%±9.95%,其促動力作用與多潘立酮相同.離體實驗:①灌流多巴胺(40 mg/ml)可明顯抑製離體結腸肌條收縮活動,較Krebs-Ringer液對照組減少91.56%±10.24%.②多潘立酮可阻斷多巴胺對離體結腸肌條的舒張作用.結論 多潘立酮可明顯增彊結腸動力,其作用明顯優于莫沙必利,併與西沙必利作用相同.多潘立酮增彊結腸動力作用是通過抗多巴胺作用實現.
목적 관찰다반립동대결장적촉동력작용,병여막사필리급서사필리비교.방법 ①재체실험:장40지대서분위대조조、다반립동조、막사필리조화서사필리조,매조10지.재각조대서근단결장화원단결장매식응력전감기,기록청성대서결장운동.②리체실험:재항온관류기조중,채용장력전감기측정다파알화다파알+다반립동대대서리체결장기조적수축활동.결과 재체실험:①청성대서재소화간기적정식상태하,결장정현절률성상위수축활동.②다반립동가명현증가결장적수축활동,사근단결장화원단결장평균진폭분별비대조조증가84.61%±7.26%화76.37%±8.47%,정제량-효응관계.동등제량적막사필리대근단결장화원단결장평균진폭분별비대조조증가50.32%±8.16%화45.13%±7.16%.막사필리대결장적촉동력작용명현저우다반립동.이동등제량적서사필리대근단결장화원단결장평균진폭분별비대조조증가92.55%±8.37%화81.27%±9.95%,기촉동력작용여다반립동상동.리체실험:①관류다파알(40 mg/ml)가명현억제리체결장기조수축활동,교Krebs-Ringer액대조조감소91.56%±10.24%.②다반립동가조단다파알대리체결장기조적서장작용.결론 다반립동가명현증강결장동력,기작용명현우우막사필리,병여서사필리작용상동.다반립동증강결장동력작용시통과항다파알작용실현.
Objective To observe the prokinetic effect of domperidone on colon and compare with that of mosapride and cisapride.Methods ① In viva experiment:forty rats were divided into control,domperidone,mosapride,and cisapride groups with 10 in each group.Stran gauges were planted both in proximal and distal colons and colonic motor activities were recoded in conscious rats.② In vitro experiment:the prokinetics effects of dopamine or dopamine combined with domperidone on the contraction of isolated rat colon strips were recorded by tone-transducers in the thermostatic muscle bath.Results In viva experiments:① in the interdigestive period of resting,activities of rhythimic phasic contraction were recoded in colon at conscious rats,and ② it had been showed that dornperidone significantly enhanced the contraction of colon in dose-dependent manner.Compared with control group,the mean contraction amplitude of proximal colon and distal colon increased by 84.61% ± 7.26% and 76.37 % ± 8.47% in domeridone group,respectively,which was higher than those in mosapride group (50.32%±8.16% and 45.13%±7.16%,respectively),but as same as those in cisapride group (92.55% ± 8.37% and 81.27% ± 9.95%,respectively).In vitro experiments:① perfusion of dopamin (40 mg/ml) could significantly decrease the contraction of isolated colon strip by 91.56% ± 10.24% in comparison with Krebs-Ringer solution,and ① domperidone could block the relaxant effect of dopamin on the isolated colon strip.Conclusions Domperidone can significantly enhance the colonic motor activities via antidopaminergic action.The effects of domperidone are similar as eisapride and greater than mosapride.
