CAJ | 학술논문

目的体外研究五株蓝舌病毒(bluetongue virus)致HeLa细胞的作用机制.方法透射电镜观察感染病毒后细胞超微结构的变化;MTT法和流式细胞仪分别检测病毒对细胞的生长和凋亡的影响;检测凋亡细胞片段化DNA和检测病毒诱导细胞后Caspase-3、-8、-9活性.结果大量细胞发生病变,出现凋亡和溶解,胞质内可见病毒包涵体及未装配成熟的亚病毒颗粒;五株病毒以浓度依赖方式抑制细胞增殖,且五株病毒的效果有差异;流式细胞仪检测可见各个凋亡阶段的凋亡细胞,并且五株蓝舌病毒感染HeLa细胞的凋亡百分比不一样;DNA-Ladder典型;Caspase-3、-8、-9活性有不同程度的升高.结论蓝舌病毒能够有效地诱导体外培养的人宫颈癌HeLa细胞进入凋亡,五株蓝舌病毒之间差异明显.
목적 체외연구오주람설병독(bluetongue virus)치HeLa세포적작용궤제.방법 투사전경관찰감염병독후세포초미결구적변화;MTT법화류식세포의분별검측병독대세포적생장화조망적영향;검측조망세포편단화DNA화검측병독유도세포후Caspase-3、-8、-9활성.결과 대량세포발생병변,출현조망화용해,포질내가견병독포함체급미장배성숙적아병독과립;오주병독이농도의뢰방식억제세포증식,차오주병독적효과유차이;류식세포의검측가견각개조망계단적조망세포,병차오주람설병독감염HeLa세포적조망백분비불일양;DNA-Ladder전형;Caspase-3、-8、-9활성유불동정도적승고.결론 람설병독능구유효지유도체외배양적인궁경암HeLa세포진입조망,오주람설병독지간차이명현.
Objective To study the death mode and mechanism of HeLa cancer cell induced by five strain bluetongue virus(BTV). Methods Transmission electron microscope(TEM) was introduced to study changes of ultrastructure. Growth and apoptosis of HeLa cell infected with bluetongue virus were detected with MTT assay and flow cytometry. DNA fragmentation and the activity of caspase-3, -8, -9 were determined by colorimetric assay. Results Many HeLa cells which infected with BTV were observed apoptosis and lyse, and in the plasma were found many viral inclubodies and subviral particles without outer layer proteins. BTV could inhibit HeLa cell proliferation moderately and different serotypes of virus had different effect. Various stages of apoptotic cells were found by flow cytometry and the percentage of apoptosis caused by five strain bluetongue virus were not the same. DNA-Ladder was typical. Caspase-3,-8 ,-9 activity were increased by varying degrees. Conclusion BTV could infect in HeLa cell efficiently and induce it to apoptosis in vitro, then different serotypes of virus have different effect.