CAJ | 학술논문

目的建立稳定、高表达红色荧光蛋白(RFP)标记的胰腺癌裸鼠原位移植瘤自发转移模型.方法将稳定、高表达RFP的人胰腺癌细胞株SW1990-RFP注射至裸鼠皮下,建立人胰腺癌裸鼠皮下移植瘤模型.通过外科手术原位移植裸鼠皮下荧光肿瘤组织小块,建立人胰腺癌裸鼠原位移植瘤自发转移模型.应用整体荧光成像系统评价人胰腺癌裸鼠原位移植瘤模型肿瘤转移及微转移的发生情况.结果成功建立12只人胰腺癌裸鼠原位移植瘤自发转移模型,建模成功率为100%.应用整体荧光成像系统可以实时监测原发肿瘤的牛长状况以及在肿瘤生长早期即可检测到各脏器微小转移的发生.结论本实验所建立的RFP标记的胰腺癌裸鼠原位移植瘤自发转移模型稳定、可靠,可以在体外无创性动态观察肿瘤的发生、发展,具较强的灵敏性及特异性.
목적 건립은정、고표체홍색형광단백(RFP)표기적이선암라서원위이식류자발전이모형.방법 장은정、고표체RFP적인이선암세포주SW1990-RFP주사지라서피하,건립인이선암라서피하이식류모형.통과외과수술원위이식라서피하형광종류조직소괴,건립인이선암라서원위이식류자발전이모형.응용정체형광성상계통평개인이선암라서원위이식류모형종류전이급미전이적발생정황.결과 성공건립12지인이선암라서원위이식류자발전이모형,건모성공솔위100%.응용정체형광성상계통가이실시감측원발종류적우장상황이급재종류생장조기즉가검측도각장기미소전이적발생.결론 본실험소건립적RFP표기적이선암라서원위이식류자발전이모형은정、가고,가이재체외무창성동태관찰종류적발생、발전,구교강적령민성급특이성.
Objective To establish a stable high red fluorescent protein (RFP)-expressing orthotopic transplantation nude mice spontaneous metastasis model of pancreatic cancer. Methods Stable high RFP-expressing cells SW1990-RFP were injected subcutaneously into mice to establish subcutaneous implantation model. Fluorescent tumor piece from subcutaneous was transplanted into the body of the pancreas to establish surgical orthotopic implantation model. The growth of primary tumor, metastasis and micrometastasis were assessed by whole-body fluorescence imaging system. Results Twelve RFP orthotopic transplantation nude mice metastasis models of pancreatic cancer were established succeeddly, the percentage of success rate was 100%. RFP-labeled pancreatic cancer growth could be monitored in real time way. The micrometastasis of primarylesions were detected in early stage with whole-body fluorescence imaging system. Concinsions The RFP orthotopic transplantation nude mice metastasis model of pancreatic cancer is stable and reliable, and can be observed dynamically in vitro in a noninvasive way,with much higher sensitivity and specificity.