CAJ | 학술논문

目的研究电针(EA)结合经颅磁刺激(rTMS)对急性脑缺血大鼠神经干细胞激活、增殖以及学习、记忆能力的影响.方法将120只雄性Wistar大鼠随机分成正常组、模型组、EA组、rTMS组和EA加rTMS组.复制急性大脑中动脉缺血模型,选取1 d作为开始治疗的时间点,分别施以EA、rTMS和EA加rTMS方法处理,大鼠在各相应时间点处死前12 h内每4 h腹腔注射溴脱氧尿嘧啶核苷(Brdu)1次,并对大鼠行电跳台试验、神经功能评分等评测,分别在治疗后的7,14,28 d进行组织切片.采用氯化三苯基四氮唑(TTC)和Brdu免疫组化方法检测,观察鼠脑梗死面积以及鼠脑Brdu阳性细胞数.结果 EA组、rTMS组和EA加rTMS组梗死侧海马齿状回颗粒细胞层(SGZ)、室管膜下层(SVZ)周围Brdu在7,14 d表达较模型组增强(P<0.05),尤其以EA加rTMS组明显,28 d各组差异无统计学意义,EA组、rTMS组和EA加rTMS组在7,14,28 d神经功能评分、电跳台试验成绩均较模型组改善(P<0.05),尤以EA加rTMS组明显.结论 EA结合rTMS治疗能促进大鼠神经干细胞的增殖和神经功能恢复,改善大鼠学习记忆能力.
목적 연구전침(EA)결합경로자자격(rTMS)대급성뇌결혈대서신경간세포격활、증식이급학습、기억능력적영향.방법 장120지웅성Wistar대서수궤분성정상조、모형조、EA조、rTMS조화EA가rTMS조.복제급성대뇌중동맥결혈모형,선취1 d작위개시치료적시간점,분별시이EA、rTMS화EA가rTMS방법처리,대서재각상응시간점처사전12 h내매4 h복강주사추탈양뇨밀정핵감(Brdu)1차,병대대서행전도태시험、신경공능평분등평측,분별재치료후적7,14,28 d진행조직절편.채용록화삼분기사담서(TTC)화Brdu면역조화방법검측,관찰서뇌경사면적이급서뇌Brdu양성세포수.결과 EA조、rTMS조화EA가rTMS조경사측해마치상회과립세포층(SGZ)、실관막하층(SVZ)주위Brdu재7,14 d표체교모형조증강(P<0.05),우기이EA가rTMS조명현,28 d각조차이무통계학의의,EA조、rTMS조화EA가rTMS조재7,14,28 d신경공능평분、전도태시험성적균교모형조개선(P<0.05),우이EA가rTMS조명현.결론 EA결합rTMS치료능촉진대서신경간세포적증식화신경공능회복,개선대서학습기억능력.
Objective To study the effect of electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on endogenous neural atem cells, as well as learning and memory in rats with cerebral ischemia. Methods One hundred and twenty male Wistar rats were randomly divided into 5 groups: a normal group, a model group, an EA group, a rTMS group and an EA plus rTMS group. After establishing acute middle cer-ebral artery occlusion, the rats in the various experimental groups were treated with EA, rTMS or EA plus rTMS. The rats' ability to avoid an electric shock was observed using a jumping stand, Immunohistochemical techniques were used to measure the infarct area and the number of Brdu-positive cells. Results A small quantity of Brdu labelled cells was observed in the subgranular zone (SGZ) and the subventricular zone (SVZ) in rats in the normal group. In the EA, rTMS and EA plus rTMS groups, the numbers of Brdu labelled cells in the SVZ and SGZ was significantly higher than in the model group after both 7 and 14 days. The expression of Brdu around the SVZ and SGZ of the 3 treatment groups increased, especially in the EA plus rTMS group. At 7, 14 and 28 d there was significant improve-ment in the neural function and electric shock avoidance test scores in the EA, rTMS and EA plus rTMS groups com-pared with the model group,with the EA plus rTMS animals scoring best. Conclusion EA plus rTMS can promote the proliferation of nerve stem cells, facilitate neurological recovery and improve learning and memory in rats after cerebral ischemia.