CAJ | 학술논문

目的探讨参芎化瘀胶囊对血管性痴呆(vascular dementia,VD)大鼠海马CA1区NF-κBp65表达的影响.方法 40只SD大鼠随机分为4组:正常组、假手术组、模型组和参芎化瘀胶囊组.采用反复夹闭双侧颈总动脉、同时腹腔注射硝普钠制备血管性痴呆大鼠模型.应用Morris水迷宫检测VD大鼠学习、记忆能力,免疫组化法检测NF-κBp65表达.结果 ①与正常组、假手术组比较,模型组海马CA1区NF-κB p65阳性细胞[(26.67±5.71)个/高倍视野]明显增多(P均＜0.01)；②与模型组比较,参芎化瘀胶囊治疗组NF-κB p65阳性细胞[(12.83±6.62)个/高倍视野]减少(P＜0.01).结论参芎化瘀胶囊可能通过减少VD大鼠脑内NF-κB p65的表达发挥脑保护作用,改善VD大鼠的学习、记忆能力.
목적 탐토삼궁화어효낭대혈관성치태(vascular dementia,VD)대서해마CA1구NF-κBp65표체적영향.방법 40지SD대서수궤분위4조:정상조、가수술조、모형조화삼궁화어효낭조.채용반복협폐쌍측경총동맥、동시복강주사초보납제비혈관성치태대서모형.응용Morris수미궁검측VD대서학습、기억능력,면역조화법검측NF-κBp65표체.결과 ①여정상조、가수술조비교,모형조해마CA1구NF-κB p65양성세포[(26.67±5.71)개/고배시야]명현증다(P균＜0.01)；②여모형조비교,삼궁화어효낭치료조NF-κB p65양성세포[(12.83±6.62)개/고배시야]감소(P＜0.01).결론 삼궁화어효낭가능통과감소VD대서뇌내NF-κB p65적표체발휘뇌보호작용,개선VD대서적학습、기억능력.
Objective To investigate the effect of Shenxiong-Huayu capsule on the expression of NF-κB p65 in CA1 area of hippocampus of vascular dementia rats.Methods 40 SD rata were evenly divided into 4 groups:normal control group,sham-operation group,model group and Shenxiong-Huayu capsule intervention group.Established vascular dementia rat models by common carotid artery repeatedly occlusion and sodium nitroprusside intraperitoneal injection.The learning and memory abilities of rats were detected by Morris water maze; the expression of NF-κB p65 was detected by immunohistochemistry.Results ① The expression of NF-κB p65 in the CA1 area of hippocampus was increased in the model group than in the sham-operation group and normal control group (P＜0.01).② The expression of NF-κB p65 in the CA1 area of hippocampus was decreased in the Shenxiong-Huayu capsule intervention group than in the model group (P＜0.01).Conclusion Shenxiong-Huayu capsule can reduce the expression of NF-κB p65 protein in CA1 area of hippocampus and improve the learning and memory abilities in vascular dementia rats.