CAJ | 학술논문

目的探讨125I粒子源照射对SD大鼠背根神经节组织形态学的影响.方法把体重150～180g的12只成年雄性SD大鼠用随机表法等分成6组；在l～6组大鼠的第5腰背根神经节周围分别植入O(钛质空壳作为健康对照组)、14.8、18.5、22.2、25.9和29.6 MBq的125I粒子源,观察大鼠的行为改变,在植入后不同时期,将大鼠分批处死,用Olympus BX51生物光学显微镜观察植入不同活度粒子,照射不同时间后,神经节及周围肌肉组织的形态学改变,并估算背根神经节受到的剂量.结果 125I粒子源植入后,大鼠的运动功能没有受到影响,7d后体重自然增长.光学显微镜结果显示,钛质空壳植入后引起个别神经节细胞轻度水肿,但核仁清晰,细胞质正常.18.5 MBq的125I粒子源植入30 d后,与植入14 d相比,细胞肿胀更明显,还出现了脱水核固缩、核碎裂现象.29.6 MBq的粒子源植入60d后大量细胞核消失、细胞核溶解、细胞质失水皱缩.同时,活度越大,粒子植入时间越长,神经节周围的肌肉组织纤维化越显著.结论受到125I粒子源的照射后,背根神经节及周围组织的损伤程度与照射强度存在剂量依赖关系.植入125I粒子源可能对各种难治性疼痛产生一定的镇痛作用.
목적 탐토125I입자원조사대SD대서배근신경절조직형태학적영향.방법 파체중150～180g적12지성년웅성SD대서용수궤표법등분성6조；재l～6조대서적제5요배근신경절주위분별식입O(태질공각작위건강대조조)、14.8、18.5、22.2、25.9화29.6 MBq적125I입자원,관찰대서적행위개변,재식입후불동시기,장대서분비처사,용Olympus BX51생물광학현미경관찰식입불동활도입자,조사불동시간후,신경절급주위기육조직적형태학개변,병고산배근신경절수도적제량.결과 125I입자원식입후,대서적운동공능몰유수도영향,7d후체중자연증장.광학현미경결과현시,태질공각식입후인기개별신경절세포경도수종,단핵인청석,세포질정상.18.5 MBq적125I입자원식입30 d후,여식입14 d상비,세포종창경명현,환출현료탈수핵고축、핵쇄렬현상.29.6 MBq적입자원식입60d후대량세포핵소실、세포핵용해、세포질실수추축.동시,활도월대,입자식입시간월장,신경절주위적기육조직섬유화월현저.결론 수도125I입자원적조사후,배근신경절급주위조직적손상정도여조사강도존재제량의뢰관계.식입125I입자원가능대각충난치성동통산생일정적진통작용.
Objective To investigate the effect of the histological changes on rat dorsal root ganglia (DRG) after 125I seed brachytherapy.Methods Twelve adult male Sprague-Dawley rats ( 150-180g each) were randomly divided into 6 groups,125I seeds with different activities of 0 (Titanium shell),14.8,18.5,22.2,25.9 and 29.6 MBq were implanted to 6 groups of rats respectively and the behavioral changes of rats were observed.The rats were killed in different periods after implantation,the morphological changes in DRG and surrounding muscle tissue were observed with an Olympus BX51 optical microscope and then the irradiation doses were estimated.Results After 125I seed implantation,the movement function of rats was not affected and the weight of rats gained after 7 days.After the titanium shell implantation,very few mild swelling was induced in neuroganglion cells that still had clear nucleolus and normal cytoplasm.At 14 days after 18.5 MBq seed implantation,cell swelling was more serious and cell dehydrating,nuclear condensation and nuclear fragmentation appeared after 30 days.At 60 days after 29.6 MBq of seed implantation,nuclear dissolution and cytoplasmic shrinkage were induced in a large number of cells.In general,the severity of fibrosis was aggravated with the time post-irradiation and the dose in the muscles around the ganglion.Conclusions After 125I seed implantation,the injury degree of DRG tissue is dose-dependent,and the 125I seed irradiation would have analgesic effect on releasing intractable pain.