中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2008年
1期
32-35
,共4页
杨胜美%彭伟%叶元华%詹瑛
楊勝美%彭偉%葉元華%詹瑛
양성미%팽위%협원화%첨영
先兆子痫%蛋白质丝氨酸苏氨酸激酶%胞间信号肽类和蛋白质类%胎盘%脐动脉
先兆子癇%蛋白質絲氨痠囌氨痠激酶%胞間信號肽類和蛋白質類%胎盤%臍動脈
선조자간%단백질사안산소안산격매%포간신호태류화단백질류%태반%제동맥
Pre-eclampsia%Protein-sefine-threonine kinases%Intercellular signaling peptides and proteins%Placenta%Umbilical artery
目的 探讨子痫前期患者胎盘和脐动脉组织中Rho相关蛋白激酶Ⅱ(RockⅡ)的表达变化及其意义.方法 采用RT-PCR、蛋白印迹法分别检测35例轻度子痫前期患者(轻度子痫前期组)、38例重度子痫前期患者(重度子痫前期组)及45例正常晚期妊娠妇女(对照组)胎盘及脐动脉组织中RockⅡmRNA及其蛋白表达水平;采用免疫组化方法 对RockⅡ在胎盘组织中的表达进行定位,B超检测脐动脉收缩期最大血流速度(S)与舒张末期血流速度(D)的比值.结果 (1)RockⅡ主要表达于胎盘合体滋养细胞的细胞质.(2)轻度子痫前期组、重度子痫前期组及对照组胎盘组织RockⅡmRNA表达水平分别为0.82±0.14、0.93±0.13及0.70 4-0.12,分别比较,差异也均有统计学意义(P＜0.01);轻度子痫前期组、重度子痫前期组及对照组胎盘组织RockⅡ蛋白表达水平分别为0.79±O.15、0.92±0.12及0.68±0.11,分别比较,差异也均有统计学意义(P＜0.01).(3)轻度子痫前期组、重度子痫前期组及对照组脐动脉组织RockⅡmRNA表达水平分别为0.69±0.13、0.55±0.12及0.76±0.10,分别比较,差异也均有统计学意义(P＜0.01);轻度子痫前期组、重度子痫前期组及对照组脐动脉组织RockⅡ蛋白表达水平分别为0.68±0.10、0.51±0.12及0.75±0.13,分别比较,差异也均有统计学意义(P＜0.01).(4)脐动脉组织RockⅡmRNA及蛋白表达水平与脐动脉S/D值均无相关性(P＞0.05).结论 RockⅡ在子痫前期患者胎盘组织中的表达水平升高,在脐动脉组织中的表达水平降低,可能参与了子痫前期的病理、生理过程.
目的 探討子癇前期患者胎盤和臍動脈組織中Rho相關蛋白激酶Ⅱ(RockⅡ)的錶達變化及其意義.方法 採用RT-PCR、蛋白印跡法分彆檢測35例輕度子癇前期患者(輕度子癇前期組)、38例重度子癇前期患者(重度子癇前期組)及45例正常晚期妊娠婦女(對照組)胎盤及臍動脈組織中RockⅡmRNA及其蛋白錶達水平;採用免疫組化方法 對RockⅡ在胎盤組織中的錶達進行定位,B超檢測臍動脈收縮期最大血流速度(S)與舒張末期血流速度(D)的比值.結果 (1)RockⅡ主要錶達于胎盤閤體滋養細胞的細胞質.(2)輕度子癇前期組、重度子癇前期組及對照組胎盤組織RockⅡmRNA錶達水平分彆為0.82±0.14、0.93±0.13及0.70 4-0.12,分彆比較,差異也均有統計學意義(P＜0.01);輕度子癇前期組、重度子癇前期組及對照組胎盤組織RockⅡ蛋白錶達水平分彆為0.79±O.15、0.92±0.12及0.68±0.11,分彆比較,差異也均有統計學意義(P＜0.01).(3)輕度子癇前期組、重度子癇前期組及對照組臍動脈組織RockⅡmRNA錶達水平分彆為0.69±0.13、0.55±0.12及0.76±0.10,分彆比較,差異也均有統計學意義(P＜0.01);輕度子癇前期組、重度子癇前期組及對照組臍動脈組織RockⅡ蛋白錶達水平分彆為0.68±0.10、0.51±0.12及0.75±0.13,分彆比較,差異也均有統計學意義(P＜0.01).(4)臍動脈組織RockⅡmRNA及蛋白錶達水平與臍動脈S/D值均無相關性(P＞0.05).結論 RockⅡ在子癇前期患者胎盤組織中的錶達水平升高,在臍動脈組織中的錶達水平降低,可能參與瞭子癇前期的病理、生理過程.
목적 탐토자간전기환자태반화제동맥조직중Rho상관단백격매Ⅱ(RockⅡ)적표체변화급기의의.방법 채용RT-PCR、단백인적법분별검측35례경도자간전기환자(경도자간전기조)、38례중도자간전기환자(중도자간전기조)급45례정상만기임신부녀(대조조)태반급제동맥조직중RockⅡmRNA급기단백표체수평;채용면역조화방법 대RockⅡ재태반조직중적표체진행정위,B초검측제동맥수축기최대혈류속도(S)여서장말기혈류속도(D)적비치.결과 (1)RockⅡ주요표체우태반합체자양세포적세포질.(2)경도자간전기조、중도자간전기조급대조조태반조직RockⅡmRNA표체수평분별위0.82±0.14、0.93±0.13급0.70 4-0.12,분별비교,차이야균유통계학의의(P＜0.01);경도자간전기조、중도자간전기조급대조조태반조직RockⅡ단백표체수평분별위0.79±O.15、0.92±0.12급0.68±0.11,분별비교,차이야균유통계학의의(P＜0.01).(3)경도자간전기조、중도자간전기조급대조조제동맥조직RockⅡmRNA표체수평분별위0.69±0.13、0.55±0.12급0.76±0.10,분별비교,차이야균유통계학의의(P＜0.01);경도자간전기조、중도자간전기조급대조조제동맥조직RockⅡ단백표체수평분별위0.68±0.10、0.51±0.12급0.75±0.13,분별비교,차이야균유통계학의의(P＜0.01).(4)제동맥조직RockⅡmRNA급단백표체수평여제동맥S/D치균무상관성(P＞0.05).결론 RockⅡ재자간전기환자태반조직중적표체수평승고,재제동맥조직중적표체수평강저,가능삼여료자간전기적병리、생리과정.
Objective To investigate the expression and significance of Rho-associated protein kinaseⅡ(Rock Ⅱ)in preeclamptic placenta and umbilical artery.Methods Semiquanfitative RT-PCR and Western blot were used to investigate the expression of RockⅡmRNA and RockⅡprotein in placenta and umbilical artery from 35 women with moderate preeclampsia(MPE group)、38 women with severepreeclampsia(SPE group)and 45 normal third trimester pregnant women(control group),the S/D value of umbilical artery was examined by ultrasound.Results (1)The expression of Rock Ⅱ mRNA of Dlacenta in MPE group(0.82±0.14)and SPE group(0.93±0.13)were signifieantly higher than that in control group (0.70 ±0.12,P＜0.01).The expression of Rock Ⅱ protein of placenta in MPE group(0.79±0.15)and SPE group(0.92±0.12)were significantly higher compared with control group(0.68±0.11,P<0.01).The expression of Rock Ⅱ mRNA and protein of placenta in SPE group were higher compared with MPE group(P＜0.01).(2)The expression of Rock Ⅱ mRNA of umbilical artery in MPE group(0.69±0.13)and SPE group(0.55±0.12)were significantly lower than that in control gmup(0.76±0.10,P＜0.01).The expression of RockⅡ protein of umbilical artery in MPE group(0.68±0.10)and SPE group(0.51±0.12)were lower compared with control group(0.75±0.13,P＜0.01).The expression of RockⅡ mRNA and protein of umbilical artery in SPE group were significantly lower compared with MPE group(P＜0.01).(3)There were no correlations between the expression of RockⅡ mRNA and protein in placenta and umbilical artery and the S/D value and birth weight(P＞0.05).Conclusion The upregulated expression of Hock Ⅱ in placentas and downregulated expression in umbilical artery may be a compensation in preeclampsia.
