CAJ | 학술논문

软骨是最早应用组织工程技术成功构建的组织之一,但由于缺乏合适的软骨构建种子细胞,因此其发展相对落后.随着干细胞研究的兴起,脂肪干细胞(ASC)因其具有分布广泛、可利用细胞量大、取材容易等优点,为ASC作为种子细胞应用于组织工程研究提供了可能;但是ASC构建软骨组织的效果却不如骨髓间充质干细胞(BMSC)理想.因此ASC在软骨组织工程中的应用仍面临着诸多问题与挑战,其中最核心的问题是如何提高ASC成软骨的效率.为此从如何纯化脂肪来源细胞、尽可能保持其中干细胞的生物学特性并优化软骨诱导方案3个方面予以综述,为提高ASC成软骨的效率提供参考.
연골시최조응용조직공정기술성공구건적조직지일,단유우결핍합괄적연골구건충자세포,인차기발전상대락후.수착간세포연구적흥기,지방간세포(ASC)인기구유분포엄범、가이용세포량대、취재용역등우점,위ASC작위충자세포응용우조직공정연구제공료가능;단시ASC구건연골조직적효과각불여골수간충질간세포(BMSC)이상.인차ASC재연골조직공정중적응용잉면림착제다문제여도전,기중최핵심적문제시여하제고ASC성연골적효솔.위차종여하순화지방래원세포、진가능보지기중간세포적생물학특성병우화연골유도방안3개방면여이종술,위제고ASC성연골적효솔제공삼고.
Cartilage is one of the earliest reconstructed tissues used in tissue engineering. Due to the lack of appropriate seeding cells, cartilage tissue engineering is, however, relatively lagged behind. With the emergence of stem cell research, adipose stem cells(ASCs) are introduced as seeding cells into tissue engineering for possessing many advantages such as wide spreading, large amount of cells available and easy to obtain. However, the outcome of tissue engineered cartilage construction by ASCs is not as ideal as that by bone marrow stem cells (BMSCs) yet. Low efficiency of ASC chondrogenesis is considered the major cause. This review summarizes the purification of adipose-derived cells, maintenance of sternness and optimization of ehondrogenie induction, which play vital roles in improving ASC s chondrogenesis.