中华医学杂志(英文版)
中華醫學雜誌(英文版)
중화의학잡지(영문판)
CHINESE MEDICAL JOURNAL
2001年
3期
248-252
,共5页
神经元cdc-2类激酶 p35nck5a kindling 癫痫 海马
神經元cdc-2類激酶 p35nck5a kindling 癲癇 海馬
신경원cdc-2류격매 p35nck5a kindling 전간 해마
cyclin dependent kinase p35nck5a kindling hippocampus neuronal plasticity
目的 检验神经元cdc-2类激酶(Cdk5/p35nck5a)在神经元成熟和萌芽中扮演着重要作用的假说。
方法 采用Western blot分析,免疫组织化学染色和免疫沉降Cdk5活性分析的方法研究了在癫痫各阶段大鼠海马区神经元cdc-2类激酶的活性变化、Cdk5和p35nck5a的表达水平及细胞内的分布。
结果 在癫痫大鼠脑中的活性比正常大鼠脑中的活性呈有意义的增高,在所有癫痫各阶段的神经元cdc-2类激酶活性中,第三阶段的活性是最高的。该激酶活性的变化与p35nck5a表达的变化是一致的。Cdk5的表达量在癫痫各阶段中是恒定不变的,但是,它在细胞内分布出现了引人注目的变化,当Cdk5活性高时Cdk5的分布从轴索转移到细胞质,p35nck5a的分布一直在细胞质内。
结论 神经元cdc-2类激酶在轴索突触再组成上扮演着重要的作用,而且,Cdk5的分布从轴索转移到细胞质的过程可能是控制其活性的一个新调控机理。
目的 檢驗神經元cdc-2類激酶(Cdk5/p35nck5a)在神經元成熟和萌芽中扮縯著重要作用的假說。
方法 採用Western blot分析,免疫組織化學染色和免疫沉降Cdk5活性分析的方法研究瞭在癲癇各階段大鼠海馬區神經元cdc-2類激酶的活性變化、Cdk5和p35nck5a的錶達水平及細胞內的分佈。
結果 在癲癇大鼠腦中的活性比正常大鼠腦中的活性呈有意義的增高,在所有癲癇各階段的神經元cdc-2類激酶活性中,第三階段的活性是最高的。該激酶活性的變化與p35nck5a錶達的變化是一緻的。Cdk5的錶達量在癲癇各階段中是恆定不變的,但是,它在細胞內分佈齣現瞭引人註目的變化,噹Cdk5活性高時Cdk5的分佈從軸索轉移到細胞質,p35nck5a的分佈一直在細胞質內。
結論 神經元cdc-2類激酶在軸索突觸再組成上扮縯著重要的作用,而且,Cdk5的分佈從軸索轉移到細胞質的過程可能是控製其活性的一箇新調控機理。
목적 검험신경원cdc-2류격매(Cdk5/p35nck5a)재신경원성숙화맹아중분연착중요작용적가설。
방법 채용Western blot분석,면역조직화학염색화면역침강Cdk5활성분석적방법연구료재전간각계단대서해마구신경원cdc-2류격매적활성변화、Cdk5화p35nck5a적표체수평급세포내적분포。
결과 재전간대서뇌중적활성비정상대서뇌중적활성정유의의적증고,재소유전간각계단적신경원cdc-2류격매활성중,제삼계단적활성시최고적。해격매활성적변화여p35nck5a표체적변화시일치적。Cdk5적표체량재전간각계단중시항정불변적,단시,타재세포내분포출현료인인주목적변화,당Cdk5활성고시Cdk5적분포종축색전이도세포질,p35nck5a적분포일직재세포질내。
결론 신경원cdc-2류격매재축색돌촉재조성상분연착중요적작용,이차,Cdk5적분포종축색전이도세포질적과정가능시공제기활성적일개신조공궤리。
Objective To test the hypothesis that neuronal cdc2-like kinase (Cdk5/p35nck5a) plays an important role in neuronal maturation and sprouting.
Methods Changes kinase activity, expression levels and subcellular localizations of Cdk5 and p35nck5a in the rat hippocampus were studied during kindling progression by Western blot analysis, immunohistochemitry, immunoprecipitation and kinase assay.
Results Kinase activity in kindling rats was significantly higher than that in normal adult rats. The kinase activity at stage 3 was most prominent among all stages of kindling progression. The changes in kinase activity coincided with those of p35nck5a expression in kindling rats. In contrast, the expression of Cdk5 was constant throughout the progression of kindling stages. However, subcellular localization of Cdk5 dramatically changed in the hippocampal neurons of kindling rats. Cdk5 was translocated from axon to soma when kinase activity was high. p35nck5a was always localized in the soma throughout kindling progression.
Conclusions Neuronal cdc2-like kinase plays an important role in synaptic reorganization, and the translocation of Cdk5 to the soma from the axon may be a novel regulatory mechanism to control kinase activity.