CAJ | 학술논문

目的观测几种肝脏疾病患者血清中糖基化磷脂酸肌醇特异性磷脂酶D(GPI-PLD)的活性改变。方法利用自制的具完整糖基化磷脂酰肌醇(GPI)结构的胎盘型碱性磷酸酶(PLAP)做底物，通过TX-114分相，定量测定血清GPI-PLD的活性，用SPSS 10.0统计软件分析资料。结果检测172例肝脏疾病患者(包括A组：26例急性重症病毒性肝炎；B组：29例肝硬化；C组：32例慢性病毒性肝炎；D组：55例急性非重症病毒性肝炎；E组：30例原发性肝癌)血清中GPI-PLD的活性(转化底物的百分率)，发现A、B两组患者的该酶活性较正常人(182名)显著性降低，而D、E两组则较正常人显著性增高，C组患者的酶活性与正常人无显著性差异，但A、B、C、D、E两组之间的酶活性水平改变却均有显著性。结论检测患者血清GPI-PLD活性水平，既可作为临床诊断急性病毒性肝炎、肝硬化、原发性肝癌等肝脏疾病的一项生化指标，也可作为这些疾病临床疗效和预后的一项判断指标。
목적관측궤충간장질병환자혈청중당기화린지산기순특이성린지매D(GPI-PLD)적활성개변。방법이용자제적구완정당기화린지선기순(GPI)결구적태반형감성린산매(PLAP)주저물，통과TX-114분상，정량측정혈청GPI-PLD적활성，용SPSS 10.0통계연건분석자료。결과검측172례간장질병환자(포괄A조：26례급성중증병독성간염；B조：29례간경화；C조：32례만성병독성간염；D조：55례급성비중증병독성간염；E조：30례원발성간암)혈청중GPI-PLD적활성(전화저물적백분솔)，발현A、B량조환자적해매활성교정상인(182명)현저성강저，이D、E량조칙교정상인현저성증고，C조환자적매활성여정상인무현저성차이，단A、B、C、D、E량조지간적매활성수평개변각균유현저성。결론검측환자혈청GPI-PLD활성수평，기가작위림상진단급성병독성간염、간경화、원발성간암등간장질병적일항생화지표，야가작위저사질병림상료효화예후적일항판단지표。
Objective　To determine the changes of serum glycosylphosphatidylinositol specific phospholipase D(GPI-PLD) activity in patients of several liver disease.Method　Glycosylphosphatidylinositol (GPI) anchored placental alkaline phosphatase(PLAP) prepared by ourselves was used as a substrate.After partitioning by triton-X-114,the serum GPI-PLD activity was determined quantitatively and the data was treated by microware of SPSS 10.0.Results　On the basis of the percentage of GPI-anchored PLAP conversion,the sera GPI-PLD activities of total 172 patients,included 26 severe acute viral hepatitis as group A,29 liver cirrhosis as group B,32 chronic viral hepatitis as group C,55 mild acute viral hepatitis as group D,30 primary hepatocellular carcinoma as group E,were measured.As compared with 182 healthy presons as control group,the sera GPI-PLD activities of group A and B were significantly reduced;By contraries,the activities of group D and E were significantly raised.The sera GPI-PLD activities of group C compared with healthy control group were not significantly altered.However,when paired Q-test,the changes of serum GPI-PLD activity between all paired groups among this five groups were remarkable.Conclusions　The determination of sera GPI-PLD activities in patients can act as a biochemistry index for diagnosis of acute viral hepatitis,liver cirrhosis and primary hepatocellular carcinoma,as well as an auxiliary index for judgment of the curative effect and prognosis of liver diseases.