CAJ | 학술논문

目的黑素细胞与同种异体淋巴细胞混合培养，从体外实验来研究排斥反应的可能性。方法采用3H-dTR掺入同位素液态闪烁计数法测定淋巴细胞的转化增殖率，并用透射电镜观察混合培养后黑素细胞的超微结构。结果黑素细胞对同种异体淋巴细胞的转化增殖率与ConA刺激淋巴细胞转化增殖的阳性对照比较，结果显示黑素细胞的促淋巴细胞转化增殖的特异性抗原作用较弱。进一步比较黑素细胞对不同病期白癜风患者淋巴细胞的影响，发现黑素细胞对活动期白癜风患者淋巴细胞的刺激作用相对较强，而稳定期患者和正常人对照组结果差异无显著性。同时电镜观察结果显示同种异体的淋巴细胞对黑素细胞无明显损伤，黑素细胞经混合培养后，细胞的形态完整，黑素合成功能正常，胞体和树突无损伤。细胞内细胞器丰富，胞浆内可见大量的不同阶段黑素小体。结论正常人黑素细胞的促淋巴细胞转化增殖的特异性抗原作用较弱。同种异体淋巴细胞对黑素细胞超微结构无破坏作用。在进行同种异体移植时最好选择稳定期患者。
목적흑소세포여동충이체림파세포혼합배양，종체외실험래연구배척반응적가능성。방법채용3H-dTR참입동위소액태섬삭계수법측정림파세포적전화증식솔，병용투사전경관찰혼합배양후흑소세포적초미결구。결과흑소세포대동충이체림파세포적전화증식솔여ConA자격림파세포전화증식적양성대조비교，결과현시흑소세포적촉림파세포전화증식적특이성항원작용교약。진일보비교흑소세포대불동병기백전풍환자림파세포적영향，발현흑소세포대활동기백전풍환자림파세포적자격작용상대교강，이은정기환자화정상인대조조결과차이무현저성。동시전경관찰결과현시동충이체적림파세포대흑소세포무명현손상，흑소세포경혼합배양후，세포적형태완정，흑소합성공능정상，포체화수돌무손상。세포내세포기봉부，포장내가견대량적불동계단흑소소체。결론정상인흑소세포적촉림파세포전화증식적특이성항원작용교약。동충이체림파세포대흑소세포초미결구무파배작용。재진행동충이체이식시최호선택은정기환자。
Objective To investigate the melanocyte-lymphocyte reacion when the allogeneic lymphocytes are cultured with normal melanocytes. Methods 3H - thymidine was incorporated into the mixed culture and the transformation and proliferation rates of lymphocytes were detected by liquid scintillation counting and expressed as cpm. Electron microscopy was used to observe the ultrastructure of melanocytes after mixed culture.Results The results of lymphocyte proliferation were expressed by the stimulation indexes. The stimulation indexes in active vitiligo group was significantly different from that in stable vitiligo group and normal controls. The stimulation indexes of the melanocyte-stimulated group was significantly different from that of ConA-stimulated group. In the mixed melanocyte-lymphocyte reaction, the allogeneic lymphocytes had little effect on the melanocytes. The ultrastructure of the melanocytes in the mixed culture showed normal morphology and normal function of synthesis of melanin. Conclusion As a specific antigen in mixed melanocyte-lymphocyte reaction, melanocyte has a weak effect on the lymphocytes. The melanocytes from stable stage vitiligo patients seem more suitble to be allografted.