CAJ | 학술논문

建立了-种固相萃取-超高效液相色谱-串联质谱法（SPE—UPLC—MS／MS）快速测定猪尿中苯乙醇胺A的方法。猪尿样品以莱克多巴胺-D，为内标，尿液经酸化后离心，上清液经OasisMCX固相萃取柱净化，超高效液相色谱-串联质谱法测定。苯乙醇胺A监测离子为m／z345〉150和m／z 345〉327。结果表明在0．2～5μg／L范围内苯乙醇胺A呈良好的线性关系，高中低浓度绝对回收率在80—110％，精密度小于15％，最低定量限0．2μg／L。本方法快速、简便，适于猪尿中苯乙醇胺A的残留测定。
건립료-충고상췌취-초고효액상색보-천련질보법（SPE—UPLC—MS／MS）쾌속측정저뇨중분을순알A적방법。저뇨양품이래극다파알-D，위내표，뇨액경산화후리심，상청액경OasisMCX고상췌취주정화，초고효액상색보-천련질보법측정。분을순알A감측리자위m／z345〉150화m／z 345〉327。결과표명재0．2～5μg／L범위내분을순알A정량호적선성관계，고중저농도절대회수솔재80—110％，정밀도소우15％，최저정량한0．2μg／L。본방법쾌속、간편，괄우저뇨중분을순알A적잔류측정。
A SPE -UPLC -MS/MS method was developed and established to rapidly determine phenylethanolamine A in pig urine. The urine sample was acidified and centrifuged, and the supernatant was cleaned up by Oasis MCX. UPLC - MS/MS was implemented to detect phenylethanolamine A by m/z 345 〉 150 and m/z 345 〉 327. The results illustrated that good linearity in the ranges of 0. 2 - 5 μg/L for phenylethanolamine A. The absolute recoveries were between 80% and 110%, and precision was lower than 15%. The limit of quantitative detection was 0.2 μg/L. The method was simple and rapid, and it was suitable to detect phenylethanolamine A residual in pig urine samples.