CAJ | 학술논문

牛的被毛颜色是重要的外貌性状。本研究采集北京地区401头荷斯坦母牛血样及21头公牛冻精样品．提取基因组DNA，选取牛6号染色体上KIT基因的4个SNPs位点，利用飞行时间质谱技术检测基因型。通过数码相机照相并进行图像分析计算牛的被毛黑白比例，记录乳头颜色。将基因型和表型数据进行关联分析，结果显示KIT基因的G72792875T住点对被毛比例有显著影响（P〈0．05），推测KIT基因可能与荷斯坦牛的着色性状的形成相关。
우적피모안색시중요적외모성상。본연구채집북경지구401두하사탄모우혈양급21두공우동정양품．제취기인조DNA，선취우6호염색체상KIT기인적4개SNPs위점，이용비행시간질보기술검측기인형。통과수마상궤조상병진행도상분석계산우적피모흑백비례，기록유두안색。장기인형화표형수거진행관련분석，결과현시KIT기인적G72792875T주점대피모비례유현저영향（P〈0．05），추측KIT기인가능여하사탄우적착색성상적형성상관。
Coat color is of importance for cattle breeding. In this study, 401 blood samples of Holstein cows and 21 frozen semen samples of bulls were collected and genomic DNA was extracted from these samples. Four SNPs of KIT gene on bovine chromosome 6 were genotyped using time-of-flight（TOF） mass spectrometry. Black coat proportion was computed from the cattle photographs. The nipple color was directly recorded. Results of association analysis showed that the SNP of KIT gene （G72792875T） has a significant effect on the black and white coat proportion（P〈0.05）, suggesting that KIT gene plays an important role in the genetic mechanism of cattle＇s pigment.