科技风
科技風
과기풍
TECHNOLOGY WIND
2011年
15期
18-19
,共2页
高效液相色谱法%(HPLC)%罗丹明B%碱性橙Ⅱ%碱性嫩黄O%苏丹红Ⅰ-Ⅳ%番茄沙司
高效液相色譜法%(HPLC)%囉丹明B%堿性橙Ⅱ%堿性嫩黃O%囌丹紅Ⅰ-Ⅳ%番茄沙司
고효액상색보법%(HPLC)%라단명B%감성등Ⅱ%감성눈황O%소단홍Ⅰ-Ⅳ%번가사사
HPLC%RhodamineB%Chrysoidine Ⅱ%AuramineO%Sudan I - IV%Tomatosauce
建立了同时测定了番茄沙司中的罗丹明B、碱性橙Ⅱ，碱性嫩黄O,苏丹红Ⅰ-Ⅳ等7种染料含量的高效液相色谱法（HPLC）。样品乙腈超声提取、浓缩过滤后，进行HPLC分离检测；结果在17min内可达到对以上七种非食用色素完全分离。试验表明：该方法的线性范围和相关系数为0.5-50mg/L和0.9993,检出限为3-10μg/kg;相对标准偏差（RSDs,n=6）为1.3％-3.0％
建立瞭同時測定瞭番茄沙司中的囉丹明B、堿性橙Ⅱ，堿性嫩黃O,囌丹紅Ⅰ-Ⅳ等7種染料含量的高效液相色譜法（HPLC）。樣品乙腈超聲提取、濃縮過濾後，進行HPLC分離檢測；結果在17min內可達到對以上七種非食用色素完全分離。試驗錶明：該方法的線性範圍和相關繫數為0.5-50mg/L和0.9993,檢齣限為3-10μg/kg;相對標準偏差（RSDs,n=6）為1.3％-3.0％
건립료동시측정료번가사사중적라단명B、감성등Ⅱ，감성눈황O,소단홍Ⅰ-Ⅳ등7충염료함량적고효액상색보법（HPLC）。양품을정초성제취、농축과려후，진행HPLC분리검측；결과재17min내가체도대이상칠충비식용색소완전분리。시험표명：해방법적선성범위화상관계수위0.5-50mg/L화0.9993,검출한위3-10μg/kg;상대표준편차（RSDs,n=6）위1.3％-3.0％
AHPLC chromatography hasbeen developed for the simultaneous quantitative analysis of odinMing B,Chrysoidine Ⅱ,Auramine O,sudan red Ⅰ-Ⅳ in Tomato .Thesample wasacetonitrile ulcrasonic extraction,concentration,filtration,for HPLC separation, detection,results can be achieved within 17min more than seven kinds of non-fbod coloring to the complete separation Under the optimal conditions the limits of detection （LODs, S/N=3）and the relative standard deviation（RSDs,N=6）for the targetcompounds were in the range of 3- 10μg/kg and 1.3%-3.0%,respectively, The calibration curve waslinear in the concentrationrange of 0.5-50mg/L with theeregression coefficientof0.0993
