CAJ | 학술논문

以红小豆资源为材料,采用硝酸还原酶测定试剂盒测定各材料硝酸还原酶含量。实验结果发现：严格按照试剂盒说明操作,普遍存在对照管值与测定管值相差不大,或对照管值大于测定管值的非正常现象。经过诱导硝酸还原酶时间梯度实验、37℃保温时间梯度实验,否认了此现象产生是由酶活性表达过低引起。将对照管中成分进行调整,消除了该现象。经讨论,认为调整后的对照比原对照更为合理。
이홍소두자원위재료,채용초산환원매측정시제합측정각재료초산환원매함량。실험결과발현：엄격안조시제합설명조작,보편존재대조관치여측정관치상차불대,혹대조관치대우측정관치적비정상현상。경과유도초산환원매시간제도실험、37℃보온시간제도실험,부인료차현상산생시유매활성표체과저인기。장대조관중성분진행조정,소제료해현상。경토론,인위조정후적대조비원대조경위합리。
Using red bean as the research material,the nitrate reductase（NR） activity was detected with a kit.The results showed that the value of the treatment was similar or even smaller than the control when operating according to the manual.Though the gradient experiments of enzyme amount and heat preservation time at 37℃,it was denied that the enzyme activity caused such phenomena.After adjusting the elements in control,this phenomenon disappeared.Therefore,the adjusting control is more reasonable.