CAJ | 학술논문

[目的】对细枝木麻黄进行组织培养和转基因研究。[方法]以耐寒性细枝木麻黄为试验材料，探讨了愈伤组织诱导、不定芽分化、农杆菌介导转化3种条件对转化效率的影响。【结果]对细枝木麻黄不定芽诱导分化适宜的激素组合为DCR＋6-BA5．0mg／L＋NAA0．5mg／L；转基因抗生素选择压力为潮霉素，共培养时间3d；以初步建立的转基因体系利用农杆菌介导法获得94株转基因木麻黄，通过PCR检测，其中61株为PCR阳性植株。[结论]该研究为细枝木麻黄的组织培养和转基因研究奠定了基础。
[목적】대세지목마황진행조직배양화전기인연구。[방법]이내한성세지목마황위시험재료，탐토료유상조직유도、불정아분화、농간균개도전화3충조건대전화효솔적영향。【결과]대세지목마황불정아유도분화괄의적격소조합위DCR＋6-BA5．0mg／L＋NAA0．5mg／L；전기인항생소선택압력위조매소，공배양시간3d；이초보건립적전기인체계이용농간균개도법획득94주전기인목마황，통과PCR검측，기중61주위PCR양성식주。[결론]해연구위세지목마황적조직배양화전기인연구전정료기출。
[Objective] The paper aimed to study the tissue culture and transformation of C. equisetifolia. [Method] C. equisetifolia were used as experimental materials to explore the effects of three conditions including callus induction, adventitious bud dif- ferentiation and Agrobacterium-mediated transformation on transformation rate of C. equisetifolia. [Result] The appropriate plant growth regulator combination on induction and differentiation of C. equisetifolia adventitious buds was DCR＋5.0 mg/L of 6-BA＋ 0.5 mg/L of NAA; hygromycin was selected for the selective pressure and co-culture time was 3 d; 94 stains of transgenic C. equisetifolia were obtained with the initially- established transgene system via Agrobacterium-mediated method, and 61 stains were PCR-positive plants according to the results of PCR detection. [Conclusion] The study had laid the foundation for tissue culture and transgene research of C. equi- setifolia.