CAJ | 학술논문

[目的]研究蝴蝶干标本DNA的提取方法。[方法]采用5种不同的方法（SDS法、SDS-巯基乙醇-酚法、CTAB法、饱和NaCl法、SDS-巯基乙醇-氯仿法）对蝴蝶干标本DNA进行提取。[结果]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法适合蝴蝶干标本基因组DNA的提取,并且能成功地应用于蝴蝶线粒体COI、EF-1α和CytB基因的PCR扩增。[结论]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法可以用于研究蝴蝶干标本DNA的抽提,为蝴蝶的分子系统进化研究奠定了基础。
[목적]연구호접간표본DNA적제취방법。[방법]채용5충불동적방법（SDS법、SDS-구기을순-분법、CTAB법、포화NaCl법、SDS-구기을순-록방법）대호접간표본DNA진행제취。[결과]SDS-구기을순-분법、포화NaCl법화SDS-구기을순-록방법괄합호접간표본기인조DNA적제취,병차능성공지응용우호접선립체COI、EF-1α화CytB기인적PCR확증。[결론]SDS-구기을순-분법、포화NaCl법화SDS-구기을순-록방법가이용우연구호접간표본DNA적추제,위호접적분자계통진화연구전정료기출。
[Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.[Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA extracted can be successfully applied to the PCR amplification of butterfly mitochondrial COI,EF-1α and CytB genes.[Conclusion]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method can be used to study the extraction of DNA from dried butterfly samples,which laid foundation for study on molecular phylogenetics of butterfly.