CAJ | 학술논문

应用RAPD技术分析了玳瑁的遗传多样性。用20个随机引物对中国南海海域玳瑁7个个体的基因组DNA进行了PCR扩增,共扩增出1 351条DNA片段,平均每个个体扩增出193条条带。在检测到的193条条带中,多态性条带为69条,多态性条带百分比为35.8%,条带大小在200 bp～3 000 bp之间,7个个体间遗传距离为0.082 9～0.1813,平均遗传距离为0.132 7±0.029 9,表明中国南海海域玳瑁的遗传多样性水平较低,应加强该区域玳瑁种质资源的保护。采用类平均聚类法（NJTREE）构建了7个个体相互关系的分子聚类图,表明该7个玳瑁个体没有形成种群的分化。
응용RAPD기술분석료대모적유전다양성。용20개수궤인물대중국남해해역대모7개개체적기인조DNA진행료PCR확증,공확증출1 351조DNA편단,평균매개개체확증출193조조대。재검측도적193조조대중,다태성조대위69조,다태성조대백분비위35.8%,조대대소재200 bp～3 000 bp지간,7개개체간유전거리위0.082 9～0.1813,평균유전거리위0.132 7±0.029 9,표명중국남해해역대모적유전다양성수평교저,응가강해구역대모충질자원적보호。채용류평균취류법（NJTREE）구건료7개개체상호관계적분자취류도,표명해7개대모개체몰유형성충군적분화。
We used RAPD software to analyze the genetic diversity of seven Hawksbill Turtles（Eretmochelys imbricata） captured in the South China Sea.We used 20 random primers to amplify 1351 fragments.On average,193 bands were amplified for each individual.Sixty - nine of 193 loci were polymorphic（35.8%）.The size of DNA fragments ranged from 200 bp to 3 000 bp.The genetic distance among individuals ranged from 0.082 9 to 0.181 3 and averaged 0.132 7±0.029 9.The phylogenetic tree of the seven turtles was constructed by NJTREE analysis using the RAPDistance 1.04 program.We conclude that the genetic diversity of Eretmochelys imbricata in the South China Sea is low.