苏州科技学院学报:自然科学版
囌州科技學院學報:自然科學版
소주과기학원학보:자연과학판
Journal of University of Science and Technology of Suzhou
2011年
4期
62-66
,共5页
何丽斯%苏家乐%刘晓青%李畅%陈尚平%项立平
何麗斯%囌傢樂%劉曉青%李暢%陳尚平%項立平
하려사%소가악%류효청%리창%진상평%항립평
高山杜鹃%干旱胁迫%光合特性%光合色素%叶绿素荧光
高山杜鵑%榦旱脅迫%光閤特性%光閤色素%葉綠素熒光
고산두견%간한협박%광합특성%광합색소%협록소형광
Rhododendron hybrides ‘Cosmopolitan’%drought stress%photosynthetic characteristics%photosynthetic pigments%chlorophyll fluorescence
以不同浓度(5%~25%)的PEG-6000模拟干旱胁迫处理高山杜鹃幼苗,研究了不同程度干旱胁迫下高山杜鹃叶片光合生理特性的变化。结果表明,干旱胁迫对高山杜鹃幼苗叶片光合和荧光参数的影响与其抗旱性密切相关,其中净光合速率(Pn)与气孔导度(Gs)的相关系数较大(r=0.988 1,n=5)。随着干旱胁迫程度加重,在高山杜鹃叶片各种光合色素含量均比对照有不同程度增加的同时,叶片的Pn、Gs、蒸腾速率(Tr)、PSⅡ最大光化学效率(Fv/Fm)、实际光化学效率(Fv′/Fm′)以及光化学猝灭系数(qP)均下降,这表明在高浓度的PEG-6000干旱胁迫下叶绿体片层中捕光Chla/b-Pro复合体已经受到损伤,光合机构受到严重破坏,明显抵消了叶片总叶绿素浓缩的综合作用。综合多个指标,分析得出:在干旱胁迫下高山杜鹃叶片净光合速率的降低主要是由非气孔因素引起的。
以不同濃度(5%~25%)的PEG-6000模擬榦旱脅迫處理高山杜鵑幼苗,研究瞭不同程度榦旱脅迫下高山杜鵑葉片光閤生理特性的變化。結果錶明,榦旱脅迫對高山杜鵑幼苗葉片光閤和熒光參數的影響與其抗旱性密切相關,其中淨光閤速率(Pn)與氣孔導度(Gs)的相關繫數較大(r=0.988 1,n=5)。隨著榦旱脅迫程度加重,在高山杜鵑葉片各種光閤色素含量均比對照有不同程度增加的同時,葉片的Pn、Gs、蒸騰速率(Tr)、PSⅡ最大光化學效率(Fv/Fm)、實際光化學效率(Fv′/Fm′)以及光化學猝滅繫數(qP)均下降,這錶明在高濃度的PEG-6000榦旱脅迫下葉綠體片層中捕光Chla/b-Pro複閤體已經受到損傷,光閤機構受到嚴重破壞,明顯牴消瞭葉片總葉綠素濃縮的綜閤作用。綜閤多箇指標,分析得齣:在榦旱脅迫下高山杜鵑葉片淨光閤速率的降低主要是由非氣孔因素引起的。
이불동농도(5%~25%)적PEG-6000모의간한협박처리고산두견유묘,연구료불동정도간한협박하고산두견협편광합생리특성적변화。결과표명,간한협박대고산두견유묘협편광합화형광삼수적영향여기항한성밀절상관,기중정광합속솔(Pn)여기공도도(Gs)적상관계수교대(r=0.988 1,n=5)。수착간한협박정도가중,재고산두견협편각충광합색소함량균비대조유불동정도증가적동시,협편적Pn、Gs、증등속솔(Tr)、PSⅡ최대광화학효솔(Fv/Fm)、실제광화학효솔(Fv′/Fm′)이급광화학졸멸계수(qP)균하강,저표명재고농도적PEG-6000간한협박하협록체편층중포광Chla/b-Pro복합체이경수도손상,광합궤구수도엄중파배,명현저소료협편총협록소농축적종합작용。종합다개지표,분석득출:재간한협박하고산두견협편정광합속솔적강저주요시유비기공인소인기적。
By adding different concentrations(5% to 25%) of polyethylene glycol(PEG-6000) into Hogaland solution to simulate the drought stress,the change of photosynthetic physiological characteristics in leaves of Rhododendron hybrides ‘Cosmopolitan’ was investigated.The results show that there is a close correlation between drought-resistance and the alteration of photosynthetic characters and chlorophyll fluorescence parameters,of which the correlation index of the net photosynthesis rate(Pn) and stomatal conductance(Gs) is higher(r=0.988 1,n=5).With increasing drought stress,the content of photosynthetic pigments increases,while Pn,Gs,transpiration rate(Tr),the maximal photochemical efficiency of PSⅡ(Fv/Fm),actual photochemical efficiency of PSⅡ(Fv′/Fm′) and photochemical quenching coefficient(qP) decreases,which demonstrates that light-harvesting chlorophyll a/b proteins in the lamella of chloroplasts are destroyed,the structures of photosystem are damaged seriously and the advantage of inspissation of photosynthetic pigments is neutralized.The comprehensive results indicate that the reduction of Pn under drought stress is mainly due to non-stomatal limitation.