CAJ | 학술논문

以保护鸡胚成纤维细胞（CEF）抵御新城疫病毒（NDV）侵袭的能力为主要检测指标，考察鸡干扰素γ（c-IFNγ）的热稳定性、酸碱稳定性及冻融耐受性。在37℃及以上的热处理中，c-IFNγ样品迅速失活，其中60℃处理1h活性即降低约70％；酸性环境中的样品极不稳定，在pH-2的液体制剂中1h，蛋白质全部失活，但在碱性制剂中，96h内其生物活性未发生明显变化；c—IFNγ液体制剂对冻融极为敏感，其生物学活性随冻融次数的增加呈线性下降。鸡干扰素γ重组蛋白对热及酸性环境不稳定，且不易冻存冻融。
이보호계배성섬유세포（CEF）저어신성역병독（NDV）침습적능력위주요검측지표，고찰계간우소γ（c-IFNγ）적열은정성、산감은정성급동융내수성。재37℃급이상적열처리중，c-IFNγ양품신속실활，기중60℃처리1h활성즉강저약70％；산성배경중적양품겁불은정，재pH-2적액체제제중1h，단백질전부실활，단재감성제제중，96h내기생물활성미발생명현변화；c—IFNγ액체제제대동융겁위민감，기생물학활성수동융차수적증가정선성하강。계간우소γ중조단백대열급산성배경불은정，차불역동존동융。
Selecting the protecting ability of resisting NDV for chicken embryo fibroblasts as the main indicator, it is studied the thermal stability, pH stability and freeze-thaw tolerance of c-IFNγ. The c-IFNγ, loses its activity rapidly in the heat treatment of 37℃ or above, and the activity can be reduced to 70 % at 60℃ for an hour. The protein which is extremely unstable in acidic environment can lose all of its activity in the liquid preparations of pH = 2 for an hour, but it has no notable change in alkaline environment within 96 hours. The liquid c-IFNγ preparations are very sensitive to the freeze-thaw, whose activity declined linearly with the increase of freeze-thaw. Chicken IFN-γ recombinant proteins, which are not suitable for freezing and thawing, are unstable in heat and acidic environment.