江苏调味副食品
江囌調味副食品
강소조미부식품
JIANGSU CONDIMENT AND SUBSIDIARY FOOD
2011年
2期
24-27
,共4页
高效液相色谱仪%柠檬黄%苯甲酸%山梨酸%最大吸收波长
高效液相色譜儀%檸檬黃%苯甲痠%山梨痠%最大吸收波長
고효액상색보의%저몽황%분갑산%산리산%최대흡수파장
HPLC%tartrazine%benzoic acid%sorbic acid%maximum absorption wavelength
为了更准确地测定食品中的柠檬黄,将样品前处理后,过0.45μm膜,采用戴安高效液相色谱仪测定,色谱柱为Luna5uC18（2）100A,250×4.6mm,柱温30.0℃,以甲醇＋0.02mol/L乙酸铵为流动相,进样量为10μL,检测波长为420nm,并同时进行了回收率的试验。结果表明,该法可消除样品中苯甲酸和山梨酸的干扰,同时减少了溶剂峰和溶剂杂质对目标物的影响,分析结果准确。
為瞭更準確地測定食品中的檸檬黃,將樣品前處理後,過0.45μm膜,採用戴安高效液相色譜儀測定,色譜柱為Luna5uC18（2）100A,250×4.6mm,柱溫30.0℃,以甲醇＋0.02mol/L乙痠銨為流動相,進樣量為10μL,檢測波長為420nm,併同時進行瞭迴收率的試驗。結果錶明,該法可消除樣品中苯甲痠和山梨痠的榦擾,同時減少瞭溶劑峰和溶劑雜質對目標物的影響,分析結果準確。
위료경준학지측정식품중적저몽황,장양품전처리후,과0.45μm막,채용대안고효액상색보의측정,색보주위Luna5uC18（2）100A,250×4.6mm,주온30.0℃,이갑순＋0.02mol/L을산안위류동상,진양량위10μL,검측파장위420nm,병동시진행료회수솔적시험。결과표명,해법가소제양품중분갑산화산리산적간우,동시감소료용제봉화용제잡질대목표물적영향,분석결과준학。
For the acute determination of tartrazine in food stuff,HPLC is applied to separate and determinate the tartrazine,The sample is firstly pretreated and then filters the 0.45 μm membrane.The chromatographic column is Luna 5 u C18（2） 100 A,250×4.6 mm and its temperature is 30.0℃.The combination of methyl alcohol plus the 0.02 mol/L ammonium acetate is taken as the mobile phase.With the injection volume of 10 μL and detection wavelength of 420 nm,the sample undergoes the test of recovery ratio.The final result points out that this method can delete the interference of benzoic acid and sorbic acid in sample,lessen the effects of solvent peak and solvent impurities to ensure its correct and acute analyzing report.
