CAJ | 학술논문

桃流胶病是一种严重危害桃树的真菌性病害,为研究PGIP基因在桃抗流胶病及抗其它真菌性病害中的作用,本研究以桃抗流胶病品种‘南京白沙’叶片为材料,对其PGIP基因及启动子序列进行克隆与分析。克隆测序获得了‘南京白沙’桃PGIP基因cDNA序列（GenBank登录号：HQ453972）和PGIP基因组DNA序列以及起始密码子上游453bp的启动子序列（GenBank登录号：HQ453974）,并将该PGIP基因命名为PpPGIP2。‘南京白沙’桃PpPGIP2序列分析显示,该DNA序列具有完整的阅读框,无内含子,与GenBank中登录的李属PGIP基因序列同源性在93%～98%之间,与测序完成的桃全基因组中该基因的序列同源性为96%;PpPGIP2编码的氨基酸序列分析显示,该氨基酸序列含有2个典型的亮氨酸重复序列,信号肽为第1～第24个氨基酸残基;PGIP基因的序列聚类图显示,除了科、属、种间的同源性差异外,桃的种内PGIP基因同源性也有较大差异;PpPGIP2启动子序列分析发现3个抗病相关元件,分别为：GT1CONSENSUS、SEBFCONSSTPR10A和WBOXATNPR1,另外还有与激素调控、胁迫有关的调控元件。本研究对‘南京白沙’桃PGIP基因和启动子的克隆与分析,将为进一步研究桃PGIP基因的表达调控及其功能分析提供参考。
도류효병시일충엄중위해도수적진균성병해,위연구PGIP기인재도항류효병급항기타진균성병해중적작용,본연구이도항류효병품충‘남경백사’협편위재료,대기PGIP기인급계동자서렬진행극륭여분석。극륭측서획득료‘남경백사’도PGIP기인cDNA서렬（GenBank등록호：HQ453972）화PGIP기인조DNA서렬이급기시밀마자상유453bp적계동자서렬（GenBank등록호：HQ453974）,병장해PGIP기인명명위PpPGIP2。‘남경백사’도PpPGIP2서렬분석현시,해DNA서렬구유완정적열독광,무내함자,여GenBank중등록적리속PGIP기인서렬동원성재93%～98%지간,여측서완성적도전기인조중해기인적서렬동원성위96%;PpPGIP2편마적안기산서렬분석현시,해안기산서렬함유2개전형적량안산중복서렬,신호태위제1～제24개안기산잔기;PGIP기인적서렬취류도현시,제료과、속、충간적동원성차이외,도적충내PGIP기인동원성야유교대차이;PpPGIP2계동자서렬분석발현3개항병상관원건,분별위：GT1CONSENSUS、SEBFCONSSTPR10A화WBOXATNPR1,령외환유여격소조공、협박유관적조공원건。본연구대‘남경백사’도PGIP기인화계동자적극륭여분석,장위진일보연구도PGIP기인적표체조공급기공능분석제공삼고。
Gummosis is one of the major fungi diseases occurred on peach tree.In order to study the role of PGIP gene playing on peach resistance to gummosis and other fungi diseases,we isolated and analysed the PGIP gene and its promoter from leaves of ＇Nanjing Baisha＇,a peach cultivar resisting gummosis.The cloning and sequencing results showed that the cDNA sequence （GenBank accession number：HQ453972） and the DNA sequence with an up-stream fragment of 453 bp length （GenBank accession number：HQ453974） from PGIP gene initiator codon of Prunus persica PGIP gene was amplified.The PGIP gene was named PpPGIP2.Sequence analysis showed that the cDNA sequence had complete open reading frame without intron,and was 93%～98% identical with the sequences of PGIP genes in Prunus and 96% identical with the genomic DNA of P.persica.The encoded amino acid sequence was a typical lecucine-repeated sequence and the predicted signal peptide was formed by 24 residues from 1 to 24.Besides family,genus and species,the clustering figure of PGIP genes showd that some diversities exsit in PGIP gene homology between peach species.Three disease resistant elements GT1CONSENSUS,SEBFCONSSTPR10A and WBOXATNPR1 were identified from PGIP promoter,which also has some hormone regulating and stress responsive elements.This study would contribute to further research on the regulation of expression and functional analysis of P.persica PGIP gene.