CAJ | 학술논문

本研究从草鱼（Ctenopharyngodon idellus）肝肾cDNA文库中克隆到草鱼cyclin G1基因。测序结果表明,草鱼cyclin G1全长cDNA为2118bp,使用ORF finder推译其开放阅读框为第256bp～第1155bp,编码299个aa,SMART在线软件预测其中N端第57个aa～第143个aa为细胞周期蛋白盒。同源性比对分析显示cyclin G1在鱼类中同源性极高,其中草鱼cyclin G1与斑马鱼的同源性为90%。通过PAML软件的密码子替换模型进行适应性进化分析,结果表明cyclin G1编码蛋白的26A、171A和225K氨基酸位点受到正选择作用。本研究为进一步了解草鱼cyclin G1结构功能与分子演化打下基础。
본연구종초어（Ctenopharyngodon idellus）간신cDNA문고중극륭도초어cyclin G1기인。측서결과표명,초어cyclin G1전장cDNA위2118bp,사용ORF finder추역기개방열독광위제256bp～제1155bp,편마299개aa,SMART재선연건예측기중N단제57개aa～제143개aa위세포주기단백합。동원성비대분석현시cyclin G1재어류중동원성겁고,기중초어cyclin G1여반마어적동원성위90%。통과PAML연건적밀마자체환모형진행괄응성진화분석,결과표명cyclin G1편마단백적26A、171A화225K안기산위점수도정선택작용。본연구위진일보료해초어cyclin G1결구공능여분자연화타하기출。
In this research,we cloned cyclin G1 gene from the liver and kideny cDNA library in grass crap.The result of sequencing showed that the full length of cDNA of grass crap cyclin G1 was 2 118 bp,and the open reading frame was 256th～1 155th bp predicted by ORF finder which could code a 299 amino acid polypeptide.The analysis result of SMART on-line software showed that N-lobe the 57th～143th aa of grass carp cyclin G1 called cyclin box was a highly conserved region.Homology alignment results showed that cyclin G1 was highly homologous in fishes,grass carp cyclin G1 shared the homology of 90% with zebrafish.The adaptive evolutionary analysis on cyclin G1 protein studying by codon substitution model in PAML software indicated that amino acid sites of 26A,171A and 225K were suffered the positive selection effect.The research lays the foundation for further studying the structure,function and molecular evolution of grass carp cyclin G1.