CAJ | 학술논문

目的探讨应用十二烷基硫酸钠（SDS）处理标本在结核分支杆菌聚合酶链反应（PCR）中的价值。方法从186例活动性结核病人收集痰、胸腹水、脑脊液、尿、血液等标本266份，标本分别用SDS和常规方法进行处理。两种方法处理的标本同时用PCR-反相膜杂交试验检测结核分支杆菌，并将结果进行比较。结果用SDS和常规方法处理的标本，PCR-反相膜杂交试验结核分支杆菌的检出率分别为65.0%（173/266）和51.5%（136/266）。通过配对计数资料卡方检验，SDS法和常规法之间的差异具有非常显著性意义（χ2=35.03，P<0.001）。结论 SDS法可以应用于临床标本结核分支杆菌PCR检测，提高结核分支杆菌PCR的检出率。
목적탐토응용십이완기류산납（SDS）처리표본재결핵분지간균취합매련반응（PCR）중적개치。방법종186례활동성결핵병인수집담、흉복수、뇌척액、뇨、혈액등표본266빈，표본분별용SDS화상규방법진행처리。량충방법처리적표본동시용PCR-반상막잡교시험검측결핵분지간균，병장결과진행비교。결과용SDS화상규방법처리적표본，PCR-반상막잡교시험결핵분지간균적검출솔분별위65.0%（173/266）화51.5%（136/266）。통과배대계수자료잡방검험，SDS법화상규법지간적차이구유비상현저성의의（χ2=35.03，P<0.001）。결론 SDS법가이응용우림상표본결핵분지간균PCR검측，제고결핵분지간균PCR적검출솔。
Objective　To evaluate specimen processing with sodium dodecyl(lauryl) sulfate(SDS) on the detection of Mycobacterium tuberculosis by PCR-reversed dot hybridization. Methods　Totally 266 specimens were collected from sputum，pleural fluid，ascites，urine or blood of patients with active tuberculosis and were treated with SDS or routine method.All specimens handled were tested for detecting M.tuberculosis by PCR-reversed dot hybridization. Results　The detection rate of M.tuberculosis from specimens prepared by SDS method was 65.0%(173/266) as compared with 51.5%(136/168) by routine method.Results from two methods were considered statistically significant (χ2=35.03,P<0.001).Conclusion　The SDS processing method may be used to detect M.tuberculosis from clinical specimens by PCR and enhances the detection rate of M.tuberculosis.