CAJ | 학술논문

获得优良的产氢菌株,鉴定并研究其产氢特性,为提高其产氢能力提供基础.对样品进行预处理和葡萄糖梯度驯化处理后,采用厌氧培养技术分离单菌落,进行菌株形态学观察、生理生化试验及16S rDNA分子生物学鉴定,并采用分批式厌氧发酵研究其产氢特性.结果表明:其中产氢最优的菌株FML-C_1为肠杆菌属细菌,葡萄糖为其产氢的最优碳源,氮源则以酵母粉和蛋白胨混合最佳,最适生长和产氢温度为35 ℃,最佳初始pH值为6.0,该条件下菌株的气相末端产物中氢体积分数达56.51%.菌株FML-C_1为一株良好的厌氧发酵产氢菌,可进一步优化其产氢工艺,以提高其产氢能力.
획득우량적산경균주,감정병연구기산경특성,위제고기산경능력제공기출.대양품진행예처리화포도당제도순화처리후,채용염양배양기술분리단균락,진행균주형태학관찰、생리생화시험급16S rDNA분자생물학감정,병채용분비식염양발효연구기산경특성.결과표명:기중산경최우적균주FML-C_1위장간균속세균,포도당위기산경적최우탄원,담원칙이효모분화단백동혼합최가,최괄생장화산경온도위35 ℃,최가초시pH치위6.0,해조건하균주적기상말단산물중경체적분수체56.51%.균주FML-C_1위일주량호적염양발효산경균,가진일보우화기산경공예,이제고기산경능력.
In order to improve the H_2 production capacity, it is necessary to select the good H_2-producing strain and research its characteristics. After pretreatment and gradient-glucose acclimation, the anaerobic culture techniques were adopted for hydrogen production bacteria screening, the analysis of morphological characteristics, physiological biochemical tests and 16S rDNA sequencing. According to the experimental results, Enterobacter FML-C_1 is identified as the best strain for H_2-producing. Glucose and the mixture of yeast and peptone are the best carbon and nitrogen sources for H_2-production, respectively. The optimal temperature is 35 ℃ and initial pH value is 6.0. The H_2 volume fraction is up to 56.51% of the total gas. Enterobacter FML-C_1 is a good stain for biohydrogen production. It is worthy of further investigation for improving its H_2 production capacity.