CAJ | 학술논문

目的探讨急性闭合性颅脑损伤患者血清Nogo-A蛋白的变化及其与颅脑创伤程度和预后的关系.方法选择31例急性闭合性颅脑损伤患者,分别于伤后第1、3,5天采集静脉血2 mL,ELISA法测定血清Nogo-A蛋白质量浓度;根据格拉斯哥昏迷评分(GCS),将患者分为轻度颅脑损伤组(n=7)、中度颅脑损伤组(n=10)和重度颅脑损伤组(n=14);根据格拉斯哥预后评分(GOS),分为预后良好组(n=23)和预后不良组(n=8).以20名健康成人作为对照组.结果重、中、轻度颅脑损伤组伤后第1、3、5天血清Nogo-A蛋白质量浓度均显著高于对照组(P<0.01).中、重度颅脑损伤组患者伤后第1、3,5天血清Nogo-A蛋白质量浓度均高于相同时间点的轻度颅脑损伤组患者(P<0.05,P<0.01).预后不良组患者伤后第1、3,5天血清Nogo-A蛋白质量浓度显著高于相同时间点的预后良好组患者(P<0.01).结论颅脑损伤后急性期患者血清Nogo-A蛋白水平显著升高,且与损伤程度及预后有一定关系.
목적 탐토급성폐합성로뇌손상환자혈청Nogo-A단백적변화급기여로뇌창상정도화예후적관계.방법 선택31례급성폐합성로뇌손상환자,분별우상후제1、3,5천채집정맥혈2 mL,ELISA법측정혈청Nogo-A단백질량농도;근거격랍사가혼미평분(GCS),장환자분위경도로뇌손상조(n=7)、중도로뇌손상조(n=10)화중도로뇌손상조(n=14);근거격랍사가예후평분(GOS),분위예후량호조(n=23)화예후불량조(n=8).이20명건강성인작위대조조.결과 중、중、경도로뇌손상조상후제1、3、5천혈청Nogo-A단백질량농도균현저고우대조조(P<0.01).중、중도로뇌손상조환자상후제1、3,5천혈청Nogo-A단백질량농도균고우상동시간점적경도로뇌손상조환자(P<0.05,P<0.01).예후불량조환자상후제1、3,5천혈청Nogo-A단백질량농도현저고우상동시간점적예후량호조환자(P<0.01).결론 로뇌손상후급성기환자혈청Nogo-A단백수평현저승고,차여손상정도급예후유일정관계.
Objective To investigate the change of serum Nogo-A protein in patients with acute closed brain injury, and explore its relationship with the severity of neuronal damage and prognosis. Methods Thirty-one patients with acute closed brain injury were enrolled. Venous blood samples (2 mL) were obtained 1, 3 and 5 d after injury. Serum concentrations of Nogo-A protein were determined by ELISA. Patients were divided into mild (n =7), moderate (n = 10) and severe (n = 14) injury groups according to Glasgow coma score (GCS), and were divided into favorable prognosis (n = 23) and poor prognosis (n = 8) groups according to Glasgow outcome score (GOS). Another 20 healthy adults were served as controls. Results The mass concentrations of serum Nogo-A protein in mild, moderate and severe injury groups 1, 3, 5 d after injury were significantly higher than those in control group (P < 0.01), and the mass concentrations of serum Nogo-A protein in moderate and severe injury groups 1, 3, 5 d after injury were significantly higher than those in mild injury group (P <0.05, P <0.01). The mass concentrations of serum Nogo-A protein 1, 3, 5 d after injury were significantly higher in poor prognosis group than those in favourable prognosis group (P < 0.01). Conclusion Serum Nogo-A protein level significantly increases after brain injury, and is related to the degree of injury and prognosis.