CAJ | 학술논문

高浓度丁醇耐受菌株是丁醇异源重组生产的关键因素.本研究对不同环境中耐受丁醇的微生物进行筛选,从自然环境中分离得到两株能够耐受高浓度丁醇的菌株,分别命名为btpz-4-1和btpz-6-3,它们耐受丁醇的浓度达到了25 g/L.通过分子标记物16S rDNA的鉴定以及分子系统进化树的分析,btpz-4-1被鉴定为Lactobacillus mucosae,btpz-6-3被鉴定为Pediococcus pentosaceus.同时,对它们的生理特性进行研究,结果显示btpz-4-1和btpz-6-3的最适生长温度分别为45℃和42℃,最适生长pH分别为6.0和6.5.
고농도정순내수균주시정순이원중조생산적관건인소.본연구대불동배경중내수정순적미생물진행사선,종자연배경중분리득도량주능구내수고농도정순적균주,분별명명위btpz-4-1화btpz-6-3,타문내수정순적농도체도료25 g/L.통과분자표기물16S rDNA적감정이급분자계통진화수적분석,btpz-4-1피감정위Lactobacillus mucosae,btpz-6-3피감정위Pediococcus pentosaceus.동시,대타문적생리특성진행연구,결과현시btpz-4-1화btpz-6-3적최괄생장온도분별위45℃화42℃,최괄생장pH분별위6.0화6.5.
Strains which can grow in high concentration of butanol is key factor for butanol production.We screened environemnt for butanol tolerant microorganisms.Two butanol tolerant strains,which was named btpz-4-1 and btpz-6-3 respectively,were isolated.They can grow in a concentration of butanol up to 25 g/L.The 16S rDNA sequences analysis of these two strains and the phylogenetic tree analysis revealed that btpz-4-1 and btpz-6-3 was Lactobacillus mucosae and Pediococcus pentosaceus,respectively.The optimal grown temperature for these two strains is 45℃ and 42℃ individually,and the optimal pH is 6.0 and 6.5.