CAJ | 학술논문

为了解小麦抗白粉病基因的组成类型以及分布状况,以加快小麦育种进程.本研究收集260份小麦作为亲本材料,根据已开发的小麦白粉病抗性基因Pm2、Pm4、Pm13和Pm21J的分子标记对其进行辅助标记鉴定.统计结果显示,260份亲本材料中,有124份材料含有Pm2单基因(占47.690/01;1份材料含有Pm13单基因(占0.38%);10份材料含有Pm2+Pm4聚合基因(占3.84%);7份材料含有Pm2+Pm13聚合基因(占2.69%);4份材料含有Pm2+Pm21聚合基因(占1.54%1;其余114份材料经上述4种标记检测没有发现相应的带型.结合田间自然诱发鉴定的结果表明:含有Pm2单基因的材料抗性不稳定,含有Pm2、Pm4、Pm13和Pm21聚合基因的材料抗性稳定,其余没有检测出上述相应标记带型的材料中也存在抗性优异的材料,但其抗病机理还需进一步研究,以期为小麦抗病育种提供新的抗源.
위료해소맥항백분병기인적조성류형이급분포상황,이가쾌소맥육충진정.본연구수집260빈소맥작위친본재료,근거이개발적소맥백분병항성기인Pm2、Pm4、Pm13화Pm21J적분자표기대기진행보조표기감정.통계결과현시,260빈친본재료중,유124빈재료함유Pm2단기인(점47.690/01;1빈재료함유Pm13단기인(점0.38%);10빈재료함유Pm2+Pm4취합기인(점3.84%);7빈재료함유Pm2+Pm13취합기인(점2.69%);4빈재료함유Pm2+Pm21취합기인(점1.54%1;기여114빈재료경상술4충표기검측몰유발현상응적대형.결합전간자연유발감정적결과표명:함유Pm2단기인적재료항성불은정,함유Pm2、Pm4、Pm13화Pm21취합기인적재료항성은정,기여몰유검측출상술상응표기대형적재료중야존재항성우이적재료,단기항병궤리환수진일보연구,이기위소맥항병육충제공신적항원.
In order to understand the composition and distribution status of powdery mildew resistance-related genes of wheat to accelerate the breeding process. In this research, we employed 260 wheat cultivars to be as parental materials for identifying the assisted marker by using four molecular markers of Pm2, Pm4, Pm13 and Pm21 gene developed from wheat powdery mildew resistance. The statistical results showed that 124 of the 260 tested materials harboring Pm2 single gene, accounting for 47.69%, only one tested material harboring Pm13 single gene, accounting for 0.38%; ten of the 260 tested materials harboring Pm2+Pm4 polymer gene, accounting for 3.84%; seven of the 260 tested materials harboring Pm2+Pm13 polymer gene, accounting for 2.69%; four of the 260 tested materials harboring Pm2+Pm21 polymer gene, accounting for 1.54%; No band-type was detected in the other 114 materials through above four marker types. And the identification of natural inducement in fields showed the instability of resistance in the tested materials harboring Pm2 single gene and the stability of resistance in the tested materials harboring polymer genes with Pm2, Pm4, Pm13 and Pm21. Among remaining materials without four kinds of marker bands, there are also few materials with good resistance to powdery mildew. However, further study on their resistance mechanism in order to provide new source of resistance for wheat breeding.